权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

網膜双極細胞の受容野形成のシナプス機構

视网膜双极细胞感受野形成的突触机制

基本信息

批准号：
02670057
负责人：
村上元彦
金额：
$ 1.41万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670057/
关键词：
網膜水平細胞 Gap junction cGMP Lーアルギニン Nitric oxide

项目摘要

【研究目的】本研究の所期の目的は,申請者らが開発したcAMP細胞内注入法によってgap junctionを遮断し,網膜双極細胞受容野形成のシナプス機構を解明することであった。ところがcGMPもまた同様な遮断効果を持つことが判明したので,急遽研究を最近トピックスとなっているnitric oxide(NO)の作用の解明に転換した。すなわちNOはcGMPの合成酵素guanylate cyclaseのactivatorであって,内皮細胞由来血管弛緩因子もNOであること,また神経系においては長期増強,記憶,神経ネットワ-クの形成などに関与すると云われており,gap junctionに対するNOの効果を明らかにすることは緊急かつ重要と考えたからである。【実験方法・結果・考察】NOはLーアルギニン:シトルリン代謝回路で発生するので,アルギニンを細胞内微小電極から電気泳動的にカメおよびコイの網膜水平細胞に注入した。少量のアルギニンの注入によってgap junctionが遮断され,細胞の入力抵抗は著明に上昇した。従って微小電極から電流を流すと細胞は容易に分極し,正確なIーV曲線を記録して,光応答の逆転電位は0mV付近と測定された。また蛍光色素Lucifer Yellowを細胞内に注入たところ,アルギニンによってdye couplingが完全に遮断されることが形態学的にも証明され,NOの作用に新たなデ-タを加えることが出来た。本法は環状ヌクレオチド注入法に比して容易に施行することが出来,将来網膜以外の神経系や非神経系組織のgap junctionの機構と,その作用の解明に広く応用されることが期待される。

[objective] the purpose of this study was to determine whether the cAMP was blocked by intracellular injection of gap junction and the formation of bipolar cellular receptive fields in the network. In order to understand the effect of cGMP, this is the same as the result of blocking. In this paper, we have made a rapid study of the role of nitric oxide (NO) in solving the problem. The synthesis of enzymes guanylate cyclase activator, endothelium-derived vasodilatation factor (NO), vascular relaxation factor (NO), vascular relaxant factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor), vascular relaxation factor (vascular relaxation factor) [the results of the investigation of the results of the experimental method] Nostradipine: the replacement circuit is used to generate DNA, and the microelectrode electrophoretic electrophoretic response to the membrane level of microelectrodes is injected into the cell. A small amount of money is injected into the gap junction to block it, and the cells try their best to resist it. It is easy to analyze the micro-electric current and current cell, and the I-V curve is recorded correctly, and the reverse electric potential 0mV is used to determine the temperature. The light pigments Lucifer Yellow were injected into the cells, and the dye coupling was completely blocked by the light pigments. The light pigments were injected into the cells of the light pigments, the light pigments were injected into the cells, and the light pigments were completely blocked by NO. In this law, it is easier to implement the environmental information injection method than the system. In the future, the external network is a non-theological organization, the gap junction organization, and its role is to explain how the system is expected to be used.