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Study of herpes viral antigen on idiotypic antibody in autoimmune disease

自身免疫性疾病独特型抗体上疱疹病毒抗原的研究

基本信息

批准号：
02670189
负责人：
SHIMIZU Yoshinobu
金额：
$ 1.41万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

When BALB/C mice(4W, *)were infected intraperitoneally with 2 x 10^5 PFU of mouse cytomegalovirus(MCMV)and the mice were breeded for 46 weeks, autoantibody was clearly shown in 16% of the MCMV infected mice after 24 weeks from infection but not in the same ages of normal mice. When the MCMV latent infected mice were administrated with anti mouse T cells antibody(alphaT), cyclospoline A(CsA)or steroid, the increased number of autoantibody positive mice was shown in the treatment of alphaT or CsA and the decreased number of that was shown in the treatment of steroid than nonadministrated mice. On the other* hand, when each spleriocytes of autoantibody positive mice and normal mice were analyzed by flowcytometry, Lyt I and L3T4 positive cells were extremely decreased in autoantibody positive mice. And also when thymocytes of normal mice were infected with MCMV, MCMV antigen was clearly exoressed in Thy 1 positive cells. . When 100 mice were infected with MCMV and analyzed the kinetics of autoantibody production and the property of autoantibody(anti nuclear antibody ; ANA and antisalivary duct antibody ; ASDA), ANA could be detected since 20 weeks after infection while ASDA since 24 weeks after infection. Their lymphoid cell infiltration could be seen in the periductal areas of submandibular glands at 20 weeks after infection and the foci could be seen all of the MCMV infected mice since 24 weeks after infection. Numbers of foci and infiltrated cells tended to increase with advancing age.These results indicated that the serologic and pathologic changes occurring in latent MCMV infected mice are very similar in respects to those which characterize Sjogren's syndrome in human.

用2×10~(-5)pfu的小鼠巨细胞病毒(MCMV)2×10~(-5)pfu腹腔感染BALB/C小鼠，饲养46周后，16%的MCMV感染小鼠在感染后24周即可检测到自身抗体，而与正常小鼠相同日龄的小鼠未检测到自身抗体。用抗小鼠T细胞抗体(AlphaT)、环磷胆碱A(CsA)或类固醇治疗MCMV潜伏感染小鼠后，AlphaT或CsA治疗组小鼠自身抗体阳性数增加，类固醇治疗组小鼠自身抗体阳性数减少。另一方面，对自身抗体阳性小鼠和正常小鼠的脾细胞进行流式细胞术分析，发现自身抗体阳性小鼠的Lyt I和L3T4阳性细胞显著减少。正常小鼠胸腺细胞感染MCMV后，Thy-1阳性细胞明显表达MCMV抗原。。当100只小鼠感染MCMV后，分析自身抗体产生的动力学和自身抗体(抗核抗体、抗涎管抗体)的性质，ANA在感染后20周即可检测到，而AsDA在感染后24周即可检测到。MCMV感染后20周，颌下腺导管周围可见淋巴样细胞浸润，24周后所有MCMV感染小鼠均可见病灶。这些结果表明，潜伏感染MCMV的小鼠的血清学和病理学变化与人类干燥综合征的特征非常相似。