Role of HTLV-I infection in development of chronic inflammatory arthropathy.

HTLV-I 感染在慢性炎症性关节病发展中的作用。

• 批准号: 02670285
• 负责人: EGUCHI Katsumi
• 金额: $ 1.34万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670285/
• 关键词: HTLV-I; chronic inflammatory arthropathy; HTLV-I-infected T cell; synovial cell; HTLV-I pX gene; protooncogene; cytokine; transfection of gene; 抗HTLVーI抗体; 合成HTLVーIエンベロップペプタイド; 血管内皮細胞

1. Infection of human synovial cells by human T-cell lymphotropic virus Type I : proliferation and granulocyte macrophage-colony stimulating factor production of synovial cells. To determine whether synovial cells are infected with HTLV-I and the infected synovial cells are able to proliferate actively and produce cytokines, the synovial cells were cocultured with HTLV-I-producing T cell lines. The synovial cells in the 5th passage after cocultivation with HTLV-I-producing T cells were shown to express the HTLV-L antigens by an immunohistochemical methods. The HTLV-I proviral DNA was also detected in the synovial cells by the polymerase chain reaction (PCR). The synovial cells by cocultivation with HTLV-I-infected T cell lines proliferate actively and transform. The proliferation of the synovial cells was essential to the contact with synovial cells and HTLV-I-producing T cell lines. Moreover, HTLV-I-infected synovial cells produced a significant amount of granulocyte macrophage-colony … More stimulating factor (GM-CSF). These results suggest that HTLV-l may cause chronic inflammetory arthropathy, as similar to that found in rheumatoid arthritis.2. Transfection of HTLV-L pX gene into U937 cells.U937 cells were transfected HTLV-L pX gene using DEAE dextran methods. U937 cells transfected HTLV-L pX gene was detected the pX MRNA by northern blotting and Tax protein by western blotting. A-IT-2 cells expressed the IL-2 Ra mRNA, but transfected U937 cells did not. However, the transfected U937 cells were detected c-fgr mRNA and p58^<c-fgr>.3. Different B-cell responses to human T-cell lymphotropic virus Type I (HTLV-I) envelope synthetic peptides in HTLV-I-infected individuals.We studied the difference in antibody activities against the viral protein and the difference in specificites of anti-HTLV-I envelope antibodies among HTLV-I-infected individuals from the same HTLV-I endemic area using a HTLV-I-gag hybrid protein and HTLV-I-env-encoded synthetic peptides as antigens, respectively. Patients with HTLV-I-infected polyarthritis made antibodies specific for VlE1 (residues 342-363) and VIE8 (191-209). Patients with HTLV-I associated myelopathy (HAM) made antibodies to the VIE7 (97-111) and VIE9 (268-286) epitopes. The activities of anti-HTLV-I antibodies in sera from HTLV-I-infected polyarthritis patients were not different from the activities of the antibodies from normal HTLV-I-carries, and no envelope peptide-specificity unique for the arthritis patients was detected. Less

1.人T细胞嗜淋巴细胞病毒I型感染人滑膜细胞：滑膜细胞增殖和粒细胞巨噬细胞集落刺激因子的产生。为了确定滑膜细胞是否被HTLV-I感染以及感染的滑膜细胞是否能够活跃地增殖并产生细胞因子，将滑膜细胞与产生HTLV-I的T细胞系共培养。免疫组化结果显示，与产生HTLV-I的T细胞共培养后的第5代滑膜细胞表达HTLV-L抗原。聚合酶链反应（PCR）检测滑膜细胞中HTLV-Ⅰ型前病毒DNA。与HTLV-I感染的T细胞系共培养的滑膜细胞增殖活跃并转化。滑膜细胞的增殖对于与滑膜细胞和产生HTLV-I的T细胞系的接触是必不可少的。此外，HTLV-I感染的滑膜细胞产生大量的粒细胞巨噬细胞集落 ...更多信息 刺激因子（GM-CSF）。这些结果表明HTLV-1可能引起慢性炎性关节病，类似于在类风湿性关节炎中发现的。HTLV-L pX基因转染U937细胞的研究：采用DEAE-dextran法将HTLV-L pX基因转染U937细胞。北方印迹法检测到pX mRNA的表达，Western印迹法检测到Tax蛋白的表达。A-IT-2细胞表达IL-2 Ra mRNA，而U937细胞不表达。转染后的U937细胞检测到c-fgr mRNA和p58 ~（<c-fgr>-1）。用HTLV-I-gag杂合蛋白和HTLV-I-env编码的合成肽作为抗原，研究了同一地区HTLV-I感染者对HTLV-I包膜蛋白的抗体活性差异和抗HTLV-I包膜抗体的特异性差异。分别患有HTLV-1感染的多关节炎的患者产生对VIE 1（残基342-363）和VIE 8（191-209）特异的抗体。HTLV-I相关脊髓病（HAM）患者产生了针对VIE 7（97-111）和VIE 9（268-286）表位的抗体。HTLV-I感染的多关节炎患者血清中的抗HTLV-I抗体的活性与正常HTLV-I携带者的抗体的活性没有差异，并且没有检测到关节炎患者特有的包膜肽特异性。少

项目成果

Katsumi Eguchi, Hiroaki Ida, Naofumi Ishikawa, Shigenobu Nagataki.: "Immunological alterations in HTLV-I carriers." Clinical Immunology. 23. 236-249 (1991)

Katsumi Eguchi、Hiroaki Ida、Naofumi Ishikawa、Shigenobu Nagataki.：“HTLV-I 携带者的免疫学改变。”

Katsuhiro Ichinose, Tatsufumi Nakamura, Atsushi Kawakami, Katsumi Eguchi, Kunihiko Nagasato, Kohji Shibayama, et al.: "Increased adherence to T-cell to human endothelial cells in patients with human T-cell lymphotropic virus type I-associated myelopathy."

Katsuhiro Ichinose、Tatsufumi Nakamura、Atsushi Kawakami、Katsumi Eguchi、Kunihiko Nagasato、Kohji Shibayama 等人：“在人类 T 细胞嗜淋巴细胞病毒 I 型相关脊髓病患者中，T 细胞对人内皮细胞的粘附增加。”

Katsumi Eguchi: "A case of adult T cell leukemia complicated by proliferative synovitis" J Rheumatol. 18. 297-299 (1991)

Katsumi Eguchi：“成人 T 细胞白血病并发增殖性滑膜炎一例”J Rheumatol。

江口 勝美: "HTLVーI感染はリウマチ性疾患か?" 医学のあゆみ. 161. 137 (1992)

Katsumi Eguchi：“HTLV-I 感染是一种风湿病吗？”医学史 161. 137 (1992)

江口 勝美: "HTLVーIキャリア-に見られる免疫異常" 臨床免疫. 23. 236-249 (1991)

Katsumi Eguchi：“HTLV-I 携带者中观察到的免疫异常”《临床免疫学》23. 236-249 (1991)。