Morphologic study on Ito cell (Fat-storing cell) contractility

伊藤细胞（储脂细胞）收缩性的形态学研究

• 批准号: 02670330
• 负责人: UENO Takato
• 金额: $ 1.54万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670330/
• 关键词: Ito cell (Fat-storing cell); contraction; hepatic microcirculation; endothelin-1; substance P; Actin; myosin; calmodulin; 肝硬変; 伊東細胞(Fatーstoring cell); 類洞内皮細胞; エンドセリンー1; サブスタンスーP; レセプタ-

We studied for the hepatic Ito cells (Fat-storing cell) contractility.1. Binding sites of ^<125>I-endothelin-1 (ET-1) and ^<125>I-substance P (SP) in the human or rat liver, which are vasoconstractive agents, were examined by light and electron microscopic auto-radiography. At the light microscopic level, a remarkable accumulation of ^<125>I-ET-1 and ^<125>I-SP grains was observed in areas which appear to be the perisinusoidal spaces. At the electron microscopic level, these grains were detected on the plasma membrane of Ito cells compared with other hepatic cells. These findings suggest that the receptors of ET-1 and SP may be present in the cells.2. Immunolocalization of actin, myosin and calmodulin in the human Ito cells was examined by immunoperoxidase methods using anti-actin, myosin and calmodulin antibodies. Immunolocalization of actin, myosin and calmodulin was observed in the cytoplasm of Ito cells. These findings suggest that Ito cells may have the contractility.3. The area of Ito cells was measured after treatment with ET-1 or SP at various concentrations in the culture medium. The area of the cells was decreased dose-dependently after treatment with ET-1 or SP. The mode of the extent and onset of the contraction was different for the two peptides. These findings suggest that Ito cells may be involved in regulation of the hepatic sinusoidal microcirculation.4. Immunolocalization of ET-1 was observed in cultured sinusoidal endothelia.5. ET-1 concentration in portal and peripheral veins was measured in patients with non-liver disease and liver cirrhosis. ET-1 concentration of these veins was higher in patients with cirrhosis than in patients with non-liver disease. The finding suggests ET-1 may be involved in the hepatic sinusoidal microcirculation.

我们研究了肝脏Ito细胞(储脂细胞)的收缩。本文用光镜和电子显微镜放射自显影技术研究了血管收缩药内皮素-1(ET-1)和P物质(SP)在人和大鼠肝脏中的结合部位。在光镜水平上，在似为尖周空隙的区域观察到明显的^&lt；125&gt；i-ET-1和^&lt；125&gt；i-SP颗粒的聚集。在电子显微镜水平上，与其他肝细胞相比，Ito细胞质膜上可检测到这些颗粒。提示ET-1和SP受体可能存在于细胞内。用抗肌动蛋白、肌球蛋白和钙调蛋白抗体，用免疫过氧化物酶方法检测肌动蛋白、肌球蛋白和钙调蛋白在人Ito细胞中的免疫定位。肌动蛋白、肌球蛋白和钙调蛋白免疫定位于Ito细胞胞浆。这些发现提示Ito细胞可能具有收缩特性。在培养液中加入不同浓度的ET-1或SP后，测量Ito细胞的面积。经ET-1或SP处理后，细胞面积呈剂量依赖性减小。两种多肽的收缩幅度和起始方式不同。提示Ito细胞可能参与了肝窦微循环的调节。观察ET-1在培养的肝窦内皮细胞中的免疫定位。本文测定了非肝病和肝硬变患者门静脉和外周静脉血中ET-1的浓度。肝硬化组血浆ET-1浓度明显高于非肝病组。提示ET-1可能参与肝窦微循环。

项目成果

Takato UENO: "Distribution of substance P and vasoactive intestinal peptide in the human liver:Light and electron immunoperoxidase methods of observation" The American Journal of Gastroenterology. 86. 1633-1637 (1991)

Takato UENO：“P物质和血管活性肠肽在人肝脏中的分布：光和电子免疫过氧化物酶观察方法”美国胃肠病学杂志。

Kazuhisa GONDO: "Endothelin-1 binding sites in rat liver tissue:Light and electron microscopic autoradiographic studies" Gastroenterology. 104. (1993)

Kazuhisa GONDO：“大鼠肝组织中的内皮素-1 结合位点：光和电子显微镜放射自显影研究”胃肠病学。

谷川 久一: "Substance P とVasoactive intestinal peptide" 肝胆膵. 21. 39-45 (1990)

谷川久一：“P 物质和血管活性肠肽”《肝胆胰》21. 39-45 (1990)。

上野 隆登: "機能 類洞壁細胞" 肝胆膵. 25. 65-73 (1992)

Takato Ueno：“肝窦壁细胞的功能”肝胆胰 25. 65-73 (1992)

Masaharu Sakamoto, Takato Ueno, Hiromasa Ohira, Takuji Torimura, Kazuhisa Gondo, Sadataka Inuzuka, Hiroshi Yoshida, Michio Sata and Kyuichi Tanikawa: "Contraction of Ito cells by endothelin-1 and substance P" Progress in Hepatic Sinusoidal Cell Studies. 5

Masaharu Sakamoto、Takato Ueno、Hiromasa Ohira、Takuji Torimura、Kazuhisa Gondo、Sadataka Inuzuka、Hiroshi Yoshida、Michio Sata 和 Kyuichi Tanikawa：“内皮素 1 和 P 物质对伊藤细胞的收缩”肝窦细胞研究进展。