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Immunological and virological study of viral myocarditis and dilated cardiomyopathy by using PCR, in situ hybridization and immunohistochemical technique

应用PCR、原位杂交和免疫组化技术对病毒性心肌炎和扩张型心肌病进行免疫学和病毒学研究

基本信息

批准号：
02670415
负责人：
DEGUCHI Hirofumi
金额：
$ 1.34万
依托单位：
Osaka midical college
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

1)Murine coxsackie B3 virus myocarditis: On days 5, 7 and 9 after virus inoculation, necrotic cardiocytes were surrounded by many CD4^+ cells and macrophages (Mac), and a few CD8^+ cells. Using in situ hybridization method, coxsackie B virus cDNA signal was detected in and around the necrotic foci. Immunoelectron microscopy revealed that virus antigen and MHC1 were present in the plasma membrane, sarcoplasmic reticulum (SR) and Golgi complex of apparently intact cardiocytes on days 5, 7, 9 and 14.CD8^+ cells were conjugated with apparently viable or degenerated cardiocytes, and MHC1 was also located on the plasma membrane of their cardiocytes. Variable expression of MHC2 was found in the cell membrane of small round cells including Mac. CD4^+ cells and Mac often infiltrated in the necrotic foci. Mac were sometimes in close contact with CD4^+ cells. It has been generally assumed that cytotoxic T cells recognize both virus antigen and MHC1, and then lyse target cells in viral infection. In our experiments MHC1 appeared to be involved in development of cardiocyte lysis by CD8^+ cells. MHC2 was detected in the membrane of Mac which were often in contact with CD4^+ cells. The contact suggested an activation of helper T cells. MHC1 and MHC2 may play an important role in antigen recognition and subsequent cell-to-cell interactions in cell-mediated immunity of myocarditis.2)Myocardial biopsies from patients with viral myocarditis and dilated cardiomyopathy(CDM): T cells and natural killer cells often infiltrated in the myocardial biopsies from patients with viral myocarditis. Many CD8^+ cells sometimes were seen in the necrotic foci. In the patients with CDM a few T cells infiltrated in the myocardium, and T4/T8 ratios in the myocardium were less than 1.0. By using PCR 32% of DCM patients demonstrated coxsackie B virus RNA signals. These findings suggest that viral infection involved in pathogenesis of DCM.

1)小鼠科萨基B3病毒性心肌炎：接种病毒后第5、7和9天，坏死的心肌细胞被许多CD 4 ^+细胞和巨噬细胞（Mac）以及少量CD 8 ^+细胞包围。用原位杂交方法检测科萨基B病毒cDNA信号。免疫电镜显示，在第5、7、9和14天，病毒抗原和MHC 1位于明显完整的心肌细胞的质膜、肌浆网（SR）和高尔基复合体上，CD 8 ^+细胞与明显存活或退化的心肌细胞结合，MHC 1也位于心肌细胞的质膜上。MHC 2在包括Mac在内的小圆细胞的细胞膜上有不同的表达。CD 4 ^+细胞和Mac常浸润于坏死灶内。Mac有时与CD 4 ^+细胞密切接触。一般认为，细胞毒性T细胞识别病毒抗原和MHC 1，然后在病毒感染中裂解靶细胞。在我们的实验中，MHC 1似乎参与了CD 8 ^+细胞溶解心肌细胞的过程。在Mac细胞膜上检测到MHC 2，Mac细胞膜常与CD 4 ^+细胞接触。这种接触表明辅助性T细胞被激活。MHC 1和MHC 2可能在心肌炎细胞免疫中的抗原识别和随后的细胞间相互作用中起重要作用。2）病毒性心肌炎和扩张型心肌病（CDM）患者的心肌活检：病毒性心肌炎患者的心肌活检组织中经常有T细胞和自然杀伤细胞浸润。坏死灶内有时可见大量CD 8 ^+细胞。CDM患者心肌内有少量T细胞浸润，心肌内T4/T8比值小于1.0。PCR检测32%的DCM患者存在科萨基B病毒RNA信号。提示病毒感染参与了DCM的发病过程。