Studies on proliferation and differentiation of human erythroblasts by phase-contrast cinemicrography

1. 脐带血来源的人红母细胞的研究(1)压缩效应：对红母细胞进行适当的压缩，以增强其精细结构的可视化，证明对其增殖和分化能力没有影响。(2)用Soret波段显微技术定性地观察了分化细胞质血红蛋白化的特征。细胞大小、运动和去核也被证明是红母细胞分化的有用指标。先天性促红细胞增生性贫血III型（CDA III）红母细胞的研究大多数CDA III红母细胞表现出与正常红母细胞相似的增殖和分化。发育不良的红母细胞的形态发生表现如下所示的两种发展模式。(1)更常见的是，形态发生开始于未成熟的红母细胞细胞核中出现一个界限清晰的区域。这个清晰的区域在子细胞和孙女细胞中再次出现。在后者中，透明区在频率、大小和硬度上都有所增加，占据了细胞核的大部分并将其分隔开。这些细胞出现了终末分化，如最大限度的血红蛋白化和剧烈的运动，细胞核呈小叶状。这些细胞似乎属于末代细胞，因为它们显示出生长的能力，并达到了大多数CDA III红母细胞分裂时的细胞大小。然而，他们并没有分裂。我们推测，这一序列可能最终导致CDA III中最常见的畸形红母细胞类型，即具有小叶或节段核的大正色正母细胞，DNA含量从2C到4C不等。(2)不太常见的有丝分裂和随后的细胞行为异常，如三极或双极有丝分裂和随后的子细胞融合，或一个子细胞的快速生长伴随着另一个子细胞的收缩。这些过程先于细胞死亡。在此过程中产生了巨大的红母细胞和一些双核红母细胞。少

1. Studies on human erythroblasts derived from cord blood(1) Effect of compression : Proper compression applied to erythroblasts to enhance visualization of their fine structures was proved to affect no influence on their capability to proliferate and differentiate.(2) Characterization of differentiation Cytoplasmic hemoglobinization was qualitatively surveyed by Soret band micrography. Cell size, movement and enucleation were also proved to be useful indices of differntiation of erythroblasts.2. Studies on Congenital dyserythropoietic anemia type III (CDA III) erythroblasts The majority of CDA III erythroblasts showed proliferation and differntiation similar to that of normal erythroblasts. Morphogenesis of dysplastic erythroblasts showed the 2 patterns of progression outlined below.(1) More frequently, morphogenesis began with the appearance of a well demarcated clear area in the nucleus of immature erythroblasts. This clear area reappeared in the daughter cells and granddaughter cel … More ls after mitosis. In the latter, the clear area, which increased in frequency, size and rigidity, occupied most of and compartmentalized the nucleus. Terminal differentiation, such as maximal hemoglobinization and vigorous movement, appeared in these cells and the nucleus became lobular. These cells seemed to belong to the end cell generation, because they showed the capacity to grow and attained the cell size at which most CDA III erythroblasts would divide. However, they did not divide. We speculate that this sequence could culminate in the most common type of dysmorphic erythroblasts in CDA III, the large orthochromatic normoblasts with lobular or segmented nucleus with DNA content ranging from 2C to 4C.(2) Less frequently, abnormalities of mitosis and subsequent cellular behavior, such as tri- or bi-polar mitosis and subsequent fusion of the daughter cells or rapid growth of one daughter cell concomitamt with shrinkage of the other daughter cell were noted. These process preceded cell death. Giant erythroblasts and some binucleate erythroblasts were apparently yielded by this process. Less