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Differentiation and maturation of human neuroblastoma into smooth muscle cells

人神经母细胞瘤向平滑肌细胞的分化和成熟

基本信息

批准号：
02670456
负责人：
SUGIMOTO Tohru
金额：
$ 1.47万
依托单位：
Kyoto Prefectural Univ. of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670456/
关键词：
nuroblastoma Smooth muscle cell Differentiation

项目摘要

The neural crest gives rise to a variety of tissues, including peripheral neurons, Schwann cells, melanocyles and ectomesencymal cells, which include the smooth muscle cells of large arteries. Cell lines derived from neuroblastoma (a neural crest tumor) have at least two distinct morphological cell types, a neuroblastic phenotype (N-type) and an epithelial-like phenotype (S-type) with characteristics of substrate-adhesiveness. We have analyzed 17 human neuroblastoma cell lines using a panel of monoclonal antibodies against cytoskeletal proteins. Three neuroblastoma cell lines (KP-N-SI, KP-N-YN and SMS-KCN) bound an alpha-smooth muscle acting antibody. In addition, one of these cell lines( KP-N-SI) bound anti-desmin monoclonal antibodies as determined by indirect immunoflourescence. A total of eight cloned cell lines were obtained from the above parent cell lines. These were composed of either N- or S-type cells and were confirmed to be the common neuroblastoma origin from each parent cell line by chromosomal analysis. Alpha-smooth muscle actin and desmin were demonstrated in the S-type cloned cells by indirect immunofluorescence, as well as by two-dimensional Western blot analysis. These results were confirmed by Northern blot analysis using a specific probe (pSHalphaSMA-3'UT) to human alpha-smooth muscle actin mRNA. This is the first report of the presence of alpha-smooth muscle actin and desmin in neuroblastoma cell lines. These data show that in addition to giving rise to cells with neural, Schwann cell and melanocyte markers, neuroblastoma can also give rise to the cells expressing smooth muscle cell marke.

神经嵴产生多种组织，包括外周神经元、许旺细胞、黑素细胞和外胚间充质细胞，其包括大动脉的平滑肌细胞。来源于神经母细胞瘤（神经嵴肿瘤）的细胞系具有至少两种不同的形态学细胞类型，神经母细胞表型（N-型）和具有基质粘附特征的上皮样表型（S-型）。我们已经分析了17人神经母细胞瘤细胞系使用一组单克隆抗体对细胞骨架蛋白。三种神经母细胞瘤细胞系（KP-N-SI、KP-N-YN和SMS-KCN）结合α-平滑肌作用抗体。此外，这些细胞系之一（KP-N-SI）结合抗结蛋白单克隆抗体，通过间接免疫荧光法测定。从上述亲本细胞系获得总共8个克隆细胞系。这些细胞由N型或S型细胞组成，并通过染色体分析证实是来自每个亲本细胞系的常见神经母细胞瘤来源。α-平滑肌肌动蛋白和结蛋白被证明在S型克隆细胞的间接免疫荧光，以及通过二维蛋白质印迹分析。这些结果通过使用针对人α-平滑肌肌动蛋白mRNA的特异性探针（pSH α SMA-3 ′ UT）的北方印迹分析来证实。这是第一次报告的存在α-平滑肌肌动蛋白和结蛋白在神经母细胞瘤细胞系。这些数据表明，除了产生具有神经、雪旺细胞和黑素细胞标记的细胞外，神经母细胞瘤还可产生表达平滑肌细胞马克标记的细胞。