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Myocardial beta-adrenoceptor Function after brain death

脑死亡后心肌β-肾上腺素受体功能

基本信息

批准号：
02670606
负责人：
TANIGUCHI Kazuhiro
金额：
$ 1.41万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670606/
关键词：
beta-adrenergic Receptor Brain Death myocardial Injury catecholamine βーアドレナリンレセプタ-

项目摘要

Post-transplant cardiac dysfunction with poor response to catecholamine may partly relate to the exogenous catecholamine used for brain-dead (BD) donor maintenance. In this experimental study using canine BD model of intracranial hypertension, alterations in myocardial beta-adrenergic receptor (BAR) were investigated. To maintain the mean arterial pressure higher than 60 mmhg after BD massive crystalloid fluid infusion (Group I) or 0.4 - 1.3 ug/kg/min of epinephrine (EPI, Group II) were used. After 3 hour (I-3hr, II-3hr) or 6 hour (I-6hr, II-6hr) of DD, blood EPI level and myocardial BAR(^<125>I-iodocyanopindlol binding) were measured.group n EPI level BAR density BAR affinity.(ng/ml) (fmol/mq) (PM)control 4 0.12<plus-minus>0.05 68.9<plus-minus>6.6 17.9<plus-minI-3hr 4 0.09<plus-minus>0.04 64.7<plus-minus>5.8 18.4<plus-minI-6hr 4 N. D. 72.3<plus-minus>6.1 18.1<plus-minusII-3hr 4 4.93<plus-minus>0.67 59.2<plus-minus>5.9 19.0<plus-minII-6hr 4 7.04<plus-minus>0.83* 41.0<plus-minus>5.0# 18.6<plus-min(* : p<0.05 vs II-3h, # : P<0.05 vs others)The result suggests that myocardial BAR density may reduce after use of high dose catecholamine in brain-dead donor and this might be partly responsible for donor heart function in heart transplantation.

移植后心功能不全对儿茶酚胺反应不良可能部分与脑死亡供体维持用外源性儿茶酚胺有关。本实验采用犬BD颅内高压模型，研究了心肌β-肾上腺素能受体（BAR）的变化。为了维持BD后的平均动脉压高于60 mmHg，使用大量晶体液输注（I组）或0.4 - 1.3 ug/kg/min肾上腺素（EPI，II组）。DD后3小时（I-3 hr，II-3 hr）或6小时（I-6 hr，II-6 hr），血EPI水平和心肌BAR（β <125>I-碘氰基吲哚洛尔结合）是measured.groupEPI水平和BAR密度的BAR亲和力。(ng/ml）（fmol/mq）（PM）对照组4 0.12 <plus-minus>0.05 68.9 <plus-minus>6.6 17.9 ± 10.09 <plus-minus>0.04 64.7 <plus-minus>5.8 ± 18.4 ± 10.04 64.7 ± 10.04 4 N。D. 72.3 <plus-minus>6.1 18.1<正-负Ⅱ-3小时4 4.93 <plus-minus>0.67 59.2 <plus-minus>5.9 19.0<正-负Ⅱ-6小时4 7.04 <plus-minus>0.83* 41.0 <plus-minus>5.0 #18.6<正-负Ⅱ（*：P<0.05 vs Ⅱ-3小时，#：P<0.05 vs其他）结果提示脑死亡供者应用大剂量儿茶酚胺后心肌BAR密度降低，这可能是心脏移植供心功能下降的部分原因。