A human chromosome 7 carries genes with putative suppressor functions for choriocarcinoma and with differentiation inducing activities for mouse EC cells.

人类 7 号染色体携带具有假定的绒毛膜癌抑制功能和小鼠 EC 细胞分化诱导活性的基因。

• 批准号: 02670747
• 负责人: MIYAMOTO Singo
• 金额: $ 0.38万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670747/
• 关键词: choriocarcinoma; Microcell fusion; Tumor suppressor genes; Chromosome #7; EC cells; Differentiation; Endoderm; Chromosome #7; 癌抑制遺伝子; 絨毛癌; 微小核融合; cDNAライブラリ-; 染色体; cDNAライブラ-

In the present study, multiple chromosomes were independently transferred into CC1 choriocarcinoma cells via microcell fusion. Tumorigenic suppression as well as alterations in growth properties were observed in the microcell hybrids only following introduction of chromosome 7. These findings were compatible with the presence of a putative tumor suppressor gene(s) for this tumor cells on chromosome 7, suggesting the availability of a single chromosome transfer to disclose a chromosome carrying a gene whose expression is critical for cell differentiation. As a result of transfer of multiple human chromosomes into mouse F9 cells, introduction of a single human chromosome 7 could induce extinction of EC cell phenotypes as well as alterations in cell morphology and growth properties of F9 cells. The microcell hybrid containing a human chromosome 7 shared biochemical markers with endoderm cells, because expression of these markers was indistinguishable from that of F9 cells differentiated with retinoic acid treatment. These results suggested that a human chromosome 7 carried genetic informations which extinguish embryonal carcinoma functions and possibly caused the EC cell genome to express biochemical and immunological characteristics of endoderm.

本研究采用微细胞融合法将多条染色体分别导入绒毛膜癌细胞CC1。仅在引入7号染色体后的微细胞杂交体中观察到致瘤抑制以及生长特性的改变。这些发现与7号染色体上存在该肿瘤细胞的假定肿瘤抑制基因相一致，表明可利用单个染色体转移来揭示携带基因的染色体，该基因的表达对细胞分化至关重要。由于将多个人染色体转移到小鼠F9细胞中，引入单个人7号染色体可诱导EC细胞表型的消失以及F9细胞的细胞形态和生长特性的改变。含有人7号染色体的微细胞杂交体与内胚层细胞共享生化标记，因为这些标记的表达与用视黄酸处理分化的F9细胞的表达不可区分。这些结果提示，人类7号染色体携带着消灭胚胎癌功能的遗传信息，并可能导致EC细胞基因组表达内胚层的生化和免疫学特性。

项目成果

Wake,N.,Yamada,H.,Fujita,H.et al: "Isolation of clones resistant to 6ーthioguanine and G418 from the HHUA endometrial carcinoma cells and their application to cell hybridization." Cancer Genetics and Cytogenetics. 49. 117-123 (1990)

Wake, N.、Yamada, H.、Fujita, H. 等人：“从 HHUA 子宫内膜癌细胞中分离出对 6-硫鸟嘌呤和 G418 具有抗性的克隆及其在细胞杂交中的应用。”49。117。 -123 (1990)

M.Sasaki,H.Yamada,Barrett,J.C.Wake,N.Oshimura,M: "Normal human chromosome 7 carries a putative suppressor gene(s) for a human choriocarcinoma cell line." Oncogene, in press.(1993)

M.Sasaki、H.Yamada、Barrett、J.C.Wake、N.Oshimura、M：“正常人类 7 号染色体携带人类绒毛膜癌细胞系的推定抑制基因。”

Sasaki, M.: "Normal human chromosome 7 carries a putative suppressor gene(s) for a human choriocarcinoma cell line." Oncogene. (1993)

Sasaki, M.：“正常人类 7 号染色体携带人类绒毛膜癌细胞系的推定抑制基因。”

H.Kato,J.Nishida,T.Honda,Y.Fujinaga,K.Fujinaga,N.Wake: "Chromosome alterations contributed to neoplastic progression of transformed rat embryonal fibroblasts." Cancer Genetics and Cytogenetics. 58. 39-47 (1992)

H.Kato、J.Nishida、T.Honda、Y.Fujinaga、K.Fujinaga、N.Wake：“染色体改变导致转化的大鼠胚胎成纤维细胞的肿瘤进展。”

和気 徳夫: "胞状奇胎からの絨毛癌化" オンコロジア. 24. 31-39 (1991)

Norio Wake：“葡萄胎引起的绒毛膜癌”《肿瘤学》24. 31-39 (1991)。