Studies on the defense mechanisms of the silkworm, Bombyx mori, against the replication of a nuclear polyhedrosis virus in continuous cell cultures

连续细胞培养中家蚕对抗核型多角体病毒复制的防御机制研究

• 批准号: 01560066
• 负责人: FUNAKOSHI Masako
• 金额: $ 1.66万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01560066/
• 关键词: defense mechanism; vaccine; nuclear polyhedrons virus; continuous cell culture; passive immunity; anti-NPV serum; interference; ウイルス不活化ワクチン; 抗血清; 抗ウイルス血清; 感染抑制; シロモンヤガ核多角体病ウイルス; 不活化ワクチン

The investigations on the defense mechanism of the silkworm against the infection of a nuclear polyhedrosis virus (NPV) have been done by means of antigenic stimulation to silkworm larvae or pupae. However, the defense reaction has not been easily demonstrated.The objective of the present study is to investigate the defense mechanism against NPV replication in Bombvx mori continuous cell cultures (S. P. C. Bm36 cells and Bm-N cells).1) The vaccines were made from the NPV (nonoccluded virus) derived from S. P. C. Bm 36 cells by treatment with formalin or irradiation with UV. The vaccine was inoculated to cultured cells 2 and 24 hr, respectively, before the inoculation of NPV. However, the induction of the defense reaction was not shown in both of S. P. C. Bm 36 cells and Bm-N cells.2) The passive immunity, , could not be recognized when the immunized rabbit serum against B. mori NPV (nonroccluded virus) derived from S. P. C. Bm 36 cells was added to the cell culture 24 and 48 hr, respectively, before the inoculation of NPV. In addition, the anti-NPV serum was added to the cell culture immediately and 60 min, respectively, after the inoculation of B. . mori NPV, however, the defense effect of the anti-NPV serum against the replication of the intracellular virus was not demonstrated.3) The interference of nonpermissive virus with the replication of permissive virus in Spodoptera frugiperda cells (IPLB-SF-21AEII) was tested. B. mori NPV which is a nonpermissive against S. frugiperda cell was inoculated to the cell culture, then after 0 and 60 min, respectively, Xestia c-nierum NPV which is permissive against S. frugiperda cell was inoculated. However, the interference of B. mori NPV with X. c-nigrum NPV was not recognized.

用抗原刺激家蚕幼虫或蛹，研究了家蚕对核型多角体病毒（NPV）感染的防御机制。本研究的目的是探讨家蚕连续培养细胞（S. mori）对NPV复制的防御机制。P. C. Bm 36细胞和Bm-N细胞）。1）疫苗由来自S. P. C.用福尔马林处理或紫外线照射Bm 36细胞。在接种NPV前2小时和24小时，将疫苗分别接种到培养的细胞中。然而，两种S均未表现出防御反应的诱导。P. C. Bm 36细胞和Bm-N细胞。2）抗B的免疫血清不能识别被动免疫。家蚕核型多角体病毒（non-roccludedvirus，NPV）是来源于家蚕的一种非封闭病毒。P. C.在接种NPV前24和48小时分别将Bm 36细胞加入细胞培养物中。另外，在接种B后立即和60 min分别向细胞培养物中加入抗NPV血清。. 3）在草地贪夜蛾（Spodoptera frugiperda）细胞（IPLB-SF-21 AEII）中，检测了非许可病毒对许可病毒复制的干扰作用。B。mori NPV对S.将草地贪夜蛾细胞接种到细胞培养物中，分别在0和60 min后，接种对草地贪夜蛾有免疫力的Xestia c-nierum NPV。接种草地早熟禾细胞。然而，B. mori NPV与X. c-nigrum NPV未被识别。