Studies on the defense mechanisms of the silkworm, Bombyx mori, against the replication of a nuclear polyhedrosis virus in continuous cell cultures

连续细胞培养中家蚕对抗核型多角体病毒复制的防御机制研究

基本信息

批准号：
01560066
负责人：
FUNAKOSHI Masako
金额：
$ 1.66万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至无数据
项目状态：
已结题

项目摘要

The investigations on the defense mechanism of the silkworm against the infection of a nuclear polyhedrosis virus (NPV) have been done by means of antigenic stimulation to silkworm larvae or pupae. However, the defense reaction has not been easily demonstrated.The objective of the present study is to investigate the defense mechanism against NPV replication in Bombvx mori continuous cell cultures (S. P. C. Bm36 cells and Bm-N cells).1) The vaccines were made from the NPV (nonoccluded virus) derived from S. P. C. Bm 36 cells by treatment with formalin or irradiation with UV. The vaccine was inoculated to cultured cells 2 and 24 hr, respectively, before the inoculation of NPV. However, the induction of the defense reaction was not shown in both of S. P. C. Bm 36 cells and Bm-N cells.2) The passive immunity, , could not be recognized when the immunized rabbit serum against B. mori NPV (nonroccluded virus) derived from S. P. C. Bm 36 cells was added to the cell culture 24 and 48 hr, respectively, before the inoculation of NPV. In addition, the anti-NPV serum was added to the cell culture immediately and 60 min, respectively, after the inoculation of B. . mori NPV, however, the defense effect of the anti-NPV serum against the replication of the intracellular virus was not demonstrated.3) The interference of nonpermissive virus with the replication of permissive virus in Spodoptera frugiperda cells (IPLB-SF-21AEII) was tested. B. mori NPV which is a nonpermissive against S. frugiperda cell was inoculated to the cell culture, then after 0 and 60 min, respectively, Xestia c-nierum NPV which is permissive against S. frugiperda cell was inoculated. However, the interference of B. mori NPV with X. c-nigrum NPV was not recognized.

关于蚕病毒感染的防御机制（NPV）的防御机制的研究是通过对蚕幼虫或p的抗原刺激进行的。然而，本研究的目的是研究防御反应的目的是调查反对反对NPV复制的防御机制（S. P. C. BM36细胞和BM-N细胞）。1）疫苗是由NPV（非封闭性病毒）生产的。在接种NPV之前，将疫苗分别接种到2和24小时的培养细胞。然而，在S. P. C. Bm 36细胞和BM-N细胞中均未显示防御反应的诱导。另外，在B.接种后，分别将抗NPV血清分别添加到细胞培养和60分钟。但是，未证明抗NPV血清对复制细胞内病毒的复制的防御作用。3）未经允许病毒在Spodoptera frugiperda细胞中的复制（IPLB-SF-21AEII）中的复制中的干扰。 B. mori nPV是针对小链球菌细胞的非允许性的，分别接种了细胞培养，然后在0和60分钟后，Xestia c-Nierum npv接种了Xestia c-Nierum npv，该Xestia c-Nierum npv被接种了针对Frugiperda细胞的允许。然而，尚未认识到X. c-npv npv的干扰。