A comprehensive study on plant molecular systematics in the higher plants based on organellar DNA analyzes

该项目已在本财政年度内完成。在这三年中，参与者进行了富有成效的合作研究，举行了会议和座谈会。(1)研究方法。为了更容易地获得被子植物的分子信息，我们试图检测PCR扩增的叶绿体DNA的几个区域的限制性内切位点变异。研究实例在Rosales（Terachi, Shimizu et al.）中显示。(2)叶绿体DNA：基于叶绿体DNA的RFLP分析和rboL基因序列的分子系统发育。两种方法得到的系统发育树的差异可归因于检测到的信息特征的数量。（清水、上田、山口）。在Eupatorieae上，通过RFLP分析（Yahara et al.）证实了二倍体属（n=10）起源于多倍体属（n=17,25）。同样，Kalimeris属被证明是多系起源，Aster ovatus被发现是Aster和Kalimeris之间的杂交，Aster是母系植物（Ito et al.）。以荞麦（Fagopyrum, Kishima et al.）为例，研究了各种栽培植物的变异和起源。芝麻（Yamada等人），土豆（Hosaka等人）和小麦（Terachi等人）。(3)线粒体DNA通过coxll和atp6基因的RFLP分析，检测到线粒体基因组的5种模式。在北海道南部和东北北部地区，其变异比其他地区更大，表明栽培G.max起源于那里（mikami et al.）。栽培Beta，苹果的变异和起源（都是Mikami等人）。稻谷（Kadowaki等人）和小麦（Mori等人）也进行了讨论。1993年9月，在日本遗传学会第65届会议上举行了一次由寺立组织的题为“高等植物分子系统学”的专题讨论会。通过本项目获得的所有成果将发表在《基于细胞器DNA分析的高等植物分子系统学综合研究》一书中。少

The profect has finished within this fiscal year. During these three years, the participants have made a fruitful co-operative study, holding assemblies and symposia.(1)Research method. For an casier way to obtain molecular information of the angiosperms, we attempted to detect restriction site variations in several regions of chloroplast DNA amplified by PCR method. The research example was shown in the Rosales(Terachi, Shimizu et al.).(2)Chloroplast DNA The molecular phylogeny in the Lardizabalaceae based on RFLP analysis of chloroplast DNA and also on sequence of rboL gene. The difference in the phylogenetic trees obtained between both methods was attributable to numbers of informative characters detected. (Shimizu, Ueda and Yamaguchi). On the Eupatorieae, the diploid genera (n=10) were proved originated from polyploid genera (n=17,25) based on RFLP analysis (Yahara et al.). Likewise, the genus Kalimeris was proved of polyphyletic origin, and Aster ovatus was found to be of hybrid o … More rigin between Aster and Kalimeris with Aster as maternal plants (Ito et al.). Variation and origin of various cultivated plants were studied with special referensces to Fagopyrum (Kishima et al.). Sesamum (Yamada et al.), potatoes (Hosaka et al.) and Triticum (Terachi et al.).(3)Mitochondrial DNA Five patterns of mitochondrial genome were detected through RFLP analysis of coxll and atp6 genes. In southern Hokkaido and northern Tohoku district, it is more variable than in other areas, suggesting origin of cultivated G.max there 8Mikami et al.). Variation and origin of the cultivated Beta, Malus(both Mikami et al.). Oryza(Kadowaki et al.) and Triticum(Mori et al.) were also discussed.In September 1993, a symposium entitled "Molecular systematics on the higher plants" organized by Terachi was held at the 65th Meeting of The Genetics Society of Japan. All the results obtained through the present project will be published in a book entitled "A comprehensive study on plant molecular systematics in the higher plants based on organellar DNA analyzes". Less