Effects of obioactin and obiopeptide-1, as a biological response modifier (BRM), on murine macrophage functions

本文研究了从抗弓形虫高免牛血清中提取的生物活性素和生物肽-1作为生物反应调节剂对小鼠巨噬细胞功能的影响。结果如下：1）体外研究：观察了生物肌动蛋白和生物肽-1对小鼠巨噬细胞葡萄糖消耗、氧中间产物释放、皱褶形成、趋化反应和杀弓形虫活性的影响。在有或没有这2种肽的情况下培养的小鼠巨噬细胞的吞噬活性和Ia阳性细胞数没有观察到差异。然而，在用这2种肽培养的小鼠巨噬细胞中，葡萄糖消耗、氧中间体释放和杀弓形虫活性显著增加。用这2种肽培养的鼠巨噬细胞的迁移也以剂量相关的方式观察到。与单独培养的巨噬细胞相比，用这两种肽培养的巨噬细胞的皱褶形成率更高。结果表明，生物素和生物肽-1具有巨噬细胞激活因子的作用，具有生物反应调节剂的特性。2）体内研究：观察两种多肽对小鼠腹腔和脾脏巨噬细胞溶酶体酶（酸性磷酸酶、β-N-乙酰葡萄糖酸苷酶、β-葡萄糖酸苷酶）活性的影响。腹腔和脾脏巨噬细胞的溶酶体酶活性均高于对照组。还在环磷酰胺或角叉菜胶预处理的小鼠中检查了对机会性感染的保护活性。这些肽显示出对一些细菌感染的保护活性。

Effects of obioactin and obiopeptide-1, as a biological response modifier, obtained from hyperimmuned bovine serum against toxoplasma gondii, on murine macrophage functions were examined. The results obtained are as follows;1) in vitro study: The changes of glucose consumption, oxygen intermediate release, ruffle formation, chemotactic responses, and toxoplasmacidal activity in murine macrophages cultivated with obioactin and obiopeptide-1 were examined. No difference was observed in phagocytic activity and Ia-positive cell numbers of murine macrophages caltivated with or without these 2 peptides. However, a significant increase of glucose consumption, oxygen intermediate release, and toxoplasmacidal activity in murine macrophage caltivated with these 2 peptides. The migration of murine macrophages caltivated with these 2 peptides was also observed at a dose related manner. The ruffle formation of macrophages caltivated with these 2 peptides were higher than those in macrophages caltivated with medium alone. These results indicated that obioactin and obiopeptide-1 have a macrophage activating factors and shows the character of biological response modifier.2) in vivo study: The changes of the lysosomal enzyme activities(acid phosphatase, beta-N-acetyl- glucronidase, beta-glucronidase) in peritoneal and splenic macrophages of mice treated with these 2 peptides were examined. The lysosomal enzyme activities in both peritoneal and splenic macrophages was higher than those in macrophages from control mice. The protective activity against opportunistic infection with bacteri was also examined in cyclophosphamide- or carrageenan-pretreated mice. These peptides showed a protective activity against some bacterial infections.