PRODUCTION OF MONOCLONAL ANTIBODIES AGAINST MAMMALIAN SUGAR-BINDING PROTEINS

67 kDa弹性蛋白结合蛋白（RL-67 EBP）已从新生大鼠肺分离使用弹性蛋白偶联亲和柱，然后用乳糖或合成弹性蛋白六肽（VGVAPG）洗脱，和免疫组织化学已被用于围产期大鼠肺，以确定该蛋白的组织定位。在胎儿肺或出生第1天和第4天新生儿肺中没有免疫反应性结构。出生后第7天，免疫反应阳性细胞出现在肺内气道的上皮下结缔组织中，从出生后第10天开始，这些细胞均匀分布在肺泡实质中。有时，一些细胞出现在肺泡气腔中，脱离肺泡隔表面。通过支气管肺泡灌洗获得的未透化细胞显示细胞表面免疫反应性，表明RL-67 EBP在细胞的表面膜上表达。从这些发现，提示免疫反应细胞是血源性单核细胞， ...更多信息 并且RL-67 EBP可以作为弹性蛋白肽受体起作用，单核细胞通过该弹性蛋白肽受体在它们从血液迁移到肺泡空气空间期间通过间质结缔组织移动，最终分化为肺泡巨噬细胞。大鼠细支气管Clara细胞的分泌颗粒根据其超微结构外观分为不同类型，然后使用抗大鼠10 kDa Clara细胞特异性蛋白进行免疫细胞化学（10 kDa CCSP）抗体。一种主要类型是椭圆形至圆形颗粒（A型颗粒），其中基质由电子密度高和电子密度低的材料的地图状混合物组成。另一种主要类型是杆状颗粒（B型颗粒）。B型颗粒的含量从细纤维状（B1型颗粒）到电子致密的棒状（B3型颗粒）结构不等。在B1和B3型颗粒之间还发现了多种中间型（B2型颗粒），在B2或B3型颗粒附近偶见小胞质囊泡。另一种类型的颗粒（C型颗粒）是大的，直径高达8 μ m，并含有适度的电子致密的无定形基质。A型和C型颗粒与抗体以相似的密度染色。新生形式的A型颗粒，这是发现在附近的高尔基体的transface，也被标记。另一方面，B型颗粒的标记密度变化：B1型颗粒几乎没有免疫标记，而B3型颗粒被强烈标记。B2型颗粒用抗体染色;然而，标记密度小于B3型颗粒。B2型颗粒的胞质小泡被标记。从这些研究结果，它表明，大鼠克拉拉细胞的颗粒由两种类型的颗粒不同的起源;一个似乎是从高尔基体起源的凝聚空泡，而另一个可能是由膜融合与未知来源的小胞质囊泡。少

67kDa elastin-binding protein (RL-67EBP) has been isolated from neonatal rat lungs by the use of an elastin-coupled affinity column, followed by elution with either lactose or synthetic elastin hexapeptide (VGVAPG), and immunohistochemistry has been used on perinatal rat lungs to determine the tissue localization of this protein. No immunoreactive structures occur in fetal lungs, or in the lungs of day-1 and -4 neonates. On day-7 after birth, immunoreactive cells appear in the subepithelial connective tissue of the intrapulmonary airways, from day-10 on, these cells become evenly distributed in the alveolar parenchyma. Occasionally, some cells occur in the alveolar air space, being free from the surface of the alveolar septum. Unpermeabilized cells obtained by bronchoalveolar lavage, show cell surface immunoreactivity, indicating that RL-67EBP is expressed on the surface membrane of the cells. From these findings, it is suggested that the immunoreactive cells are blood-borne monocytes, … More and that RL-67EBP may function as an elastin peptide receptor by which monocytes mobilize through interstitial connective tissue during their migration from blood to alveolar air space, where they eventually differentiate into alveolar macrophages.The secretory granules of rat bronchiolar Clara cells were classified into different types by their ultrastructural appearances followed by immunocytochemistry using anti-rat 10 kDa Clara cell-specific protein (10 kDa CCSP) antibody. One predominant type was the oval to round granule (type A granule), of which the matrix was composed of a map-like mixture of electron-dense and less electron-dense material. Another predominant type was the rod-shaped granule (type B granule). The content of type B granules varied from a finely fibrillar (type B1 granule) to an electron-dense, rod-like (type B3 granule) structure. Various intermediate types (type B2 granule) between type B1 and B3 granules were also found. Small cytoplasmic vesicles were found occasionally in close proximity to type B2 or B3 granule. Another type of granule (type C granule) was large, up to 8 mum in diameter, and contained a moderately electron-dense amorphous matrix. Both type A and C granules stained at a similar density with the anti-body. The nascent form of type A granules, which was found in the vicinity to the trans face of the Golgi apparatus, was also labeled. On the other hand, the labeling density of type B granules varied: type B1 granules were almost devoid of immunolabeling, whereas type B3 granules were intensely labeled. Type B2 granules stained with the antibody; however, the labeling density was less than that of type B3 granules. The small cytoplasmic vesicles of type B2 granules were labeled. From these findings, it is suggested that the granules of rat Clara cells consist of two types of granules of distinct origin; one appears to derive from condensing vacuoles of Golgi origin, whereas the other may be formed by membrane-fusions with small cytoplasmic vesicles of unknown source. Less