内因性急死における血液化学検査の法医診断学的応用

血液化学检测在内源性猝死中的法医诊断应用

• 批准号: 03670308
• 负责人: 武市 早苗
• 金额: $ 0.77万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670308/
• 关键词: 死体血液; 血液化学検査; 正常値; 参考値; 死後経過時間; 溶血度

法医解剖時に右心房から採取した血液100余例について血液化学検査を行ない、生体における血液化学検査の正常値に相当する死体血液の参考値を設定した。血液採取の条件は15〜60才の男女で死後経過時間が24時間以内のもの,死亡前に治療や救命処置の行なわれていないもの,溶血のめやすとしての血漿Hb値が0.20g/dl以下のものとした。ほとんどの測定項的において死体血液参考値の範囲は生体血液正常値より広く、死体血液の値のバラツキの大きさに起因するものと考えられた。この段階で腎機能検査のスクリ-ニングには応用可能であることがわかった。しかしこの値を腎機能スクリ-ニング以外の法医診断に用いるためにはさらに検討を行なう必要があるので、参考値を実用化するための予備試験として、ラットを用いて各項目の死後経過時間による変動を測定したところ、死体血液とほぼ同一の傾向が認められた。すなわちLDH,GOT,GPTは溶血により値が大きく変動し、CPK,GLUは死後経過時間に伴い値の大きな上昇あるいは低下が認められ、TG、UA、T、BIL,CREでは徐々に上昇傾向がみられた。Ca,ALB,BUNは死後数時間では変動は認められなかったが、その後上昇あるいは低下傾向を示し、TP、T、CHOは大きな変化は認められなかった。したがって死後の変動の大きい項目については、さらに溶血度別あるいは死後経過時間別の参考値を設定することによって法医診断に実用可能なものになると考えられた。また、死後経過や溶血に伴う値の上昇の著しいCPK,LDHについては異常高値を示すものの、アイソザイムを測定することによって心疾患の診断に応用可能であると考えられるため、現在検討中である。

During forensic autopsy, blood samples from the right atrium were collected from the right atrium in more than 100 cases. Blood chemical tests were performed in more than 100 cases. Blood chemical tests were performed in healthy volunteers. Normal blood chemistry was similar to that in dead blood samples. The condition of blood sampling was 15: 60 before men and women were killed within 24 hours after death. Emergency treatment was performed before death. Emergency treatment was performed before death. Hemolysis assay was used to determine the number of blood samples. Hb levels were measured below 0.20g/dl. In the range of reference for dead blood, the blood of living body is normal, that of dead body is normal, and that of dead blood is not normal. The remote remote machine is able to make sure that it is possible to use the remote phone to make sure that it is possible. The medical examiner can use the medical examiner to use the medical examiner to obtain the necessary medical information, to refer to the medical equipment to use the medical equipment, and to use the medical equipment to determine the safety after the death of each item. after the death of each item, the blood samples of the dead body will be tested in the same way. After the death of LDH,GOT,GPT, there was a lot of exercise in the hospital. After the death of CPK,GLU, there was a short time after the death of the drug. After the death of the drug, it was accompanied by the release of the drug, TG, UA, T, BIL,CRE, and so on. A few hours after the death of the Ca,ALB,BUN, it was possible to send a message to the audience, TP, T, CHO, and then to the audience, TP, T, and DOS. After the death of a doctor, do a lot of work, do a lot of work, do something about hemolysis, and then, after the death of a doctor, please refer to the setting of the device. Do not allow the forensic medical examiner to use the possible medical records. After he died, he was killed by hemolysis, and after death, he was told that he was suffering from heart disease, and that he might be sick after death, and now he is suffering from heart disease. now he is suffering from heart disease.