Diagnosis of Rejection in Renal Transplantation Recipient by Reactivity of Peripheral Mononuclear Cells to IL-2

白介素2(IL-2)被认为能引起细胞的有效增殖，这是导致同种异体移植排斥反应的原因。检测外周血单个核细胞(PBMN)对重组白介素2(rIL-2)的反应性，以监测肾移植受者的免疫状态。对24名处于稳定状态的患者进行研究。11例患者在发生排斥反应时进行了研究。对8名患者在稳定状态和排斥状态下进行了研究。另外，对9例健康对照和7例慢性肾功能衰竭血液透析患者进行了对照研究。以2.5U/mlIL-2(IL-2-LR)与PBMN孵育90h后，用~(3 H)掺入法检测PBMN的反应性。此外，以K562、Daudi和供体PBL为靶细胞，通过铬释放法检测扩增细胞的细胞毒作用。为了研究参与这一反应的细胞的特征，采用几种组合的双色流式细胞仪对细胞表面标志物进行分析。

Interleukin-2 (IL-2) is thought to evoke potent expansion of cells which are responsible for allograft rejection. The response of peripheral blood mononuclear cells(PBMN) to recombinant IL-2 (rIL-2) was examined in order to monitor the host immunological state in renal allograft recipients. Twenty-four patients were studied at the stable state. Eleven patients were studied at the time of rejection. Eight patients were studied both at the stable state and at the rejection. In addition, 9 healthy controls and 7 patients under hemodialysis for chronic renal failure were also studied for comparison. The response of PBMN were evaluated by ^3H uptake after a 90-hour-incubation with rIl-2 at the concentration of 2.5 U/ml (IL-2-LR). In addition, Cytotoxicity of the expanded cells were measured by Cr^<51>Cr release using K562, Daudi and donor PBL as target cells. In order to investigate the characteristics of cells participating in this reaction, the surface markers of cells were analyzed by two-color flow cytometric evaluation using several combinations.