Cellular Responses of Human Periodontal Ligament Fibroblasts by the Mechanical Stress

机械应力对人牙周膜成纤维细胞的细胞反应

• 批准号: 03670878
• 负责人: KAWASE Toshio
• 金额: $ 1.34万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670878/
• 关键词: Mechanical Stress; Humand Periodontal Ligament Fibroblasts; Cell Growth; Cell Differentiation; Alkaline Phosphatase; Cell Attachment and Spreading Factors; Stretching

Periodontium is always stressed by the mastication or oclussion which might be expressed with the several categories of mechanical stresses. In this research project, we have focuesed on the cell growth and differentiation, and cell attachment and spreading factors (CASFs) of the fibroblasts derived from human periodontal ligaments of first premolars as descrived by Kawase, T et al (1988). The CASFs which may promote the regeneration of periodontium were dramatically synthesized by the mechanical stretching. The characterization of CASFs were investgated by the following experiments.1. The CASFs in the conditioned medium of HPLF(HPLF-CM) were not inhibited by Gly-Arg-Asp-Ser peptide containing RGD cell attachment signal domain.2. The CASFs of HPLF-CM did not affect polyclonal antibodies against fibronectin(FN), vitronectin(VN) and their receptors and could bind with hydroxyapatite.3. The HPLF-CM did not contain only CASFs, but also inhibitors to CASF activity which were regulated by growth factors.We conclude that mechanical stress may play an important role in the cell proliferation and differentiation, and also could regulate the production of extracellular matrix such as CASFs.

牙周组织常受到咀嚼或咬合的应力作用，而咀嚼或咬合所受的应力可分为几类。本课题主要研究了Kawase，T等（1988）报道的人第一前磨牙牙周韧带成纤维细胞的生长、分化及细胞粘附和扩散因子（cellattachmentandspreadingfactor，CASFs）。机械牵张法可显著合成具有促进牙周组织再生作用的CASFs。通过以下实验对CASF的表征进行了研究.含RGD细胞粘附信号结构域的Gly-Arg-Asp-Ser肽对HPLF条件培养基（HPLF-CM）中的CASFs无抑制作用. HPLF-CM的CASF不影响抗纤维连接蛋白（FN）、玻连蛋白（VN）及其受体的多克隆抗体，并能与羟基磷灰石结合. HPLF-CM中不仅含有CASFs，还含有受生长因子调控的CASF活性抑制剂，提示机械应力可能在细胞增殖和分化中起重要作用，并可调控CASFs等细胞外基质的产生。

项目成果

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加藤义雄：“人牙周膜成纤维细胞的细胞粘附和扩散因子 - 细胞生长因子的调节”神奈川牙科科学 27（3）（1992）。

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Sakamoto，Takafumi 等人：“来自人牙周膜成纤维细胞条件培养基的细胞附着和扩散因子的部分纯化”Bull Kanakawa Dent Coll。

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Yasu N.: "Effect of cyclic mechanical stress on attachment and Spreading of human periodontal ligament Fibroblasts" Bull of Kanagawa Dent.Col.20(1). 30-37 (1992)

Yasu N.：“循环机械应力对人牙周膜成纤维细胞附着和扩散的影响”Bull of Kanakawa Dent.Col.20(1)。