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The Effect of Chemotherapy on The Local Environment in The Pocket

化疗对口袋局部环境的影响

基本信息

批准号：
03670906
负责人：
FUKUDA Mitsuo
金额：
$ 1.34万
依托单位：
Aichi Gakuin University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670906/
关键词：
Antibiotics Local Environment pH 〓ポケット内pH 細菌叢連続嫌気培養歯周病原細菌

项目摘要

The pH was measured in untreated periodontally involved pockets as one of environmental factors. The relation between pH and subgingival flora was determined. The range of pH in periodontal pocket was distributed between 6.23 and 8.68 and mean was 7.66. The mean ph of the low pH pockets was 6.92 and 8.53 in the high pH pockets. Gram-positive bacteria such as Streptococcus and Actynomyces comprised about 60% of the total isolated from the low pH pockets. In the pocket of high pH, gram-negative rods comprised about 60% of the total isolated bacteria. In the continuous mix culture of P.gingivalis and A.viscosus or S.milleri at pH 7.0, P.gingivalis did not show enough growth in the presence of A.viscosus or S.milleri. The pH in micro diffusion chambers for P.gingivalis and A.Viscosus was changed from 7.0 after 24 h. The pH in chambers for P.gingivalis was 8.02, pH 6.75 for A.viscosus.It is suggested that the antagonistic relationship between P.gingivalis and A.viscosus or Streptococcus may play a pathological role in theprogression of periodontal disease, and the change in bacterial flora in adult periodontilis was retated to the shift in pH in periodontal pockets.To evaluate the effect of pH on the susceptibility of antibiotics, 5 antibiotics (Tetracycline, Minocycline, Doxycycline, Clindamycin and Lincomycin) were analyzed by determinating their minimum inhibitory concentration against 9 periodontitis-associated bacterial strains. As for Doxycycline and Minocycline, the MIC values were less influensed by pH in comparison with Tetracycline. The MIC value of Clindamycin had a tendency to be less than that of Clindamycin.

在未经治疗的牙周病涉及袋作为环境因素之一的pH值进行了测量。测定pH值与龈下植物群的关系。牙周袋内pH值范围为6.23 ~ 8.68，平均值为7.66。低pH值袋的平均pH值为6.92，高pH值袋的平均pH值为8.53。链球菌和Actynomyces等革兰氏阳性细菌约占从低pH口袋中分离出的总数的60%。在高pH的口袋中，革兰氏阴性杆菌占总分离细菌的约60%。在pH7.0的牙龈卟啉单胞菌与粘性放线菌或米勒链球菌的连续混合培养中，牙龈卟啉单胞菌在粘性放线菌或米勒链球菌存在下没有显示足够的生长。牙龈卟啉单胞菌和粘性放线菌微扩散池的pH值在24 h后从7.0开始变化。牙龈卟啉单胞菌和粘性放线菌的pH值分别为8.02和6.75，提示牙龈卟啉单胞菌与粘性放线菌或链球菌之间的拮抗关系在牙周病的发展中可能起病理作用，牙周袋内细菌植物群的变化与牙周袋内pH值的变化有关。通过测定5种抗生素（四环素、米诺环素、强力霉素、克林霉素和林可霉素）对9种牙周炎相关细菌的最低抑菌浓度来分析它们。与四环素相比，强力霉素和米诺环素的MIC值受pH的影响较小。克林霉素的MIC值有小于克林霉素的趋势。