Chemical Structure and Physiological Function of Hydrophobic Molecule-binding Protein in Cytosols

细胞质中疏水分子结合蛋白的化学结构和生理功能

• 批准号: 03680143
• 负责人: ODANI Shoji
• 金额: $ 1.22万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03680143/
• 关键词: fatty acid binding protein; affinity labeling; rat liver; protein chemistry; chemical carcinogen; azo-dye; amino acid sequence; kidney; タンパク質の一次構造; 生体防御; 翻訳後修飾; 脂肪酸

Hydrophobic molecule-binding proteins in cytosols were investigated for their structure and function relationship and for cellular distribution.1. A novel molecular species of rat liver fatty acid binding protein having isoaspartyl residue at position 105 was identified and its physiological significance was investigated.2. Photo-sensitive affinity labels were synthesized and used for identification of hydrophobic ligand-binding site(s) in fatty acid binding protein. Oleoylimidazolide was also synthesized and shown to be an effective affinity-label for fatty acid binding region.3. A hydrophobic carcinogenic dye, amino-azobenzene, was administered to rats and binding site for its metabolically activated product was identified. Two other binding proteins were also identified.4. A new hydrophobic molecule-binding protein was isolated from intestinal epithelium and fully sequenced.5. Two fatty acid binding proteins were isolated from rat kidneys. It was shown that one of them is a member of lipocalin superfamily and the other heart type fatty acid binding protein.

研究了细胞质中疏水性分子结合蛋白的结构和功能关系以及细胞分布。结果：1.鉴定了一种新的大鼠肝脏脂肪酸结合蛋白，其第105位含有异天冬氨酸残基，并研究了其生理意义。合成了光敏亲和标记，并用于鉴定脂肪酸结合蛋白中疏水配体结合位点(S)。合成了油酸咪唑类化合物，并证明其是一种有效的脂肪酸结合区亲和标记。将一种疏水致癌染料氨基偶氮苯作用于大鼠，确定了其代谢活性产物的结合部位。另外还鉴定了两个结合蛋白。从肠道上皮细胞中分离到一种新的疏水性分子结合蛋白，并进行了全序列测定。从大鼠肾脏中分离到两种脂肪酸结合蛋白。结果表明，其中一个是Lipocalin超家族成员，另一个是心脏型脂肪酸结合蛋白。

项目成果

Kiumra,Hideki: "Primary structure and cellular distribution of two fatty acid binding proteins in adult rat kidneys." Journal of Biological Chemistry. 266(6). 5963-5972 (1991)

Kiumra，Hideki：“成年大鼠肾脏中两种脂肪酸结合蛋白的一级结构和细胞分布。”

Kimura, Hideki: "Primary structure and cellular distribution of two fatty acid-bindin protein in adult rat kidneys" Journal of Biological Chemistry. 266. 5963-5972 (1991)

Kimura, Hideki：“成年大鼠肾脏中两种脂肪酸结合蛋白的一级结构和细胞分布”生物化学杂志。

KINURA,Hideko: "PRIMARY STRUCTURE AND CELLULAR DISTRIBUTION OF TWO FATTY ACID-BINDING PROTEIN IN ADULT RAT KIDNEYS" JOURNAL OF BIOLOGICAL CHEMISTRY. 266. 5963-5972 (1991)

KINURA，Hideko：“成年大鼠肾脏中两种脂肪酸结合蛋白的主要结构和细胞分布”生物化学杂志。

KIMURA,HIDEKI: "PRIMARY STRUCTURE AND CELLULAR DISTRIBUTION OF TWO FATTY ACID-BINDING PROTEIN IN ADULT RAT KIDNEYS" JOURNAL OF BIOLOGICAL CHEMISTRY. 266. 5963-5972 (1991)

木村秀树：“成年大鼠肾脏中两种脂肪酸结合蛋白的初级结构和细胞分布”生物化学杂志。

KANDA,TATSUO: "PRIMARY STRUCTURE OF A 15-kDa PROTEIN FROM RAT INTESTINAL EPITHELIUM:SEQUENCE SIMILARITY TO FATTY ACID-BINDING PROTEINS" EUROPEAN JOURNAL OF BIOCHEMISTRY. 197. 759-768 (1991)

KANDA, Tatsuo：“来自大鼠肠上皮的 15 kDa 蛋白质的主要结构：与脂肪酸结合蛋白的序列相似性”欧洲生物化学杂志。