Development of trunk injection agents for a pine-wilt desease

点击翻译按钮获取中文摘要

Dehydrosinefungin is the potent nematicidal compound, but the productivity of this compound by Strepromyces sp. H-114 is very low. Thus, an improvement of the productivity is needed. We developed the quantitative method for dehydrosinefungin to find the best conditions for production of this compound. Paired-ion chromatography using an HPLC aparatus resulted inthe best separation of dehydrosinefungin and its analogs, sinefungin and amidinosinefungin. Guided by the productivity of dehydrosinefungin, we determined the best culture conditions. Using the best conditions, the productivity reached more than 20 mg/l, that is, ten times as high as that of original conditions. Large scale cultivation using a jar-fermentor gave us more than 200 mg of pure dehydrosinefugin. This enabled us to carry out the assay using three-year pine trees.On the other hand, we established the method for a trunk injection of the active compound using morantel tartarate as a test material. Using this method, dehydrosinefungin was injected to three-year pine trees before the treatment of nematodes. We found that 0.5 mg dehydrosinefungin per a three-year pine tree resulted in the resistance of tested trees against wilting. This result indicated that dehydrosinefungin was the promising agent for a pine-wilt desease.