Development of two-dimensional measurement system of intracellular potential using the voltage sensitive fluorescent dye.

使用电压敏感荧光染料开发细胞内电位二维测量系统。

• 批准号: 03557077
• 负责人: NOZAKI Shuuichi
• 金额: $ 4.16万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03557077/
• 关键词: Voltage sensitive fluorescent dye; Intracellular potential; High sensitivity image sensor; High speed CCD video camera; Two-dimensional measurement system; CCD素子

The two-dimensional measurement system of the intracellular potential using fluorescent dye is a PC controlled image monitoring system. The system takes its input from a high sensitivity camera and store the image in an image memory. This data can then be viewed in various formats on a video monitor or a PC monitor.The HARDWARE SPECIFICATIONS of the test for the system of the intracellular potential measurement system using fluorescent dye are as follows.1. Sensor type : XY address type imaging device.2. Pixel number : 256X2563. Pixel size : 40mum*.4. Resolution : 256(H)X256(V)5. Noise figure : -56dB6. Flame speed : 4500/sec(250X250)-40500/sec(64X64)7. Maximum flame number : 30008. Minimum bright sensitivity : 0.01luxThe conditions under which data is to be input from the camera and stored are set from the controller PC by the operator. Normally the system waits for a trigger signal (either internal or external) before storing data to the image memory. The input data from the camera unit is differentially amplified and averaged before being stored.The image memory data can then be filtered and displayed on the video monitor or sent directly over the General Purpose Interface Bus (GPIB) to the controller PC where it is displayed in various formats on the PC monitor.Image memory data can be saved to disk or video, and previously stored data can be reloaded from disk onto the system.

荧光染料法细胞内电位二维测量系统是一个由PC机控制的图像监控系统。该系统从高灵敏度相机获取其输入并将图像存储在图像存储器中。这些数据可以在视频监视器或PC监视器上以各种格式查看。使用荧光染料的细胞内电位测量系统的系统测试的硬件规格如下。1.传感器类型：XY地址型成像设备.像素数：256 X2563。像素尺寸：40 μ m * 0.4。分辨率：256（H）X256（V）5.噪声系数：-56dB6。火焰速度：4500/秒（250 X250）-40500/秒（64 X64）7.最大火焰数：30008。最小亮度灵敏度：0.01lux从摄像机输入数据和存储数据的条件由操作员从控制器PC设置。通常，系统在将数据存储到图像存储器之前等待触发信号（内部或外部）。来自摄像机单元的输入数据在存储之前被差分放大和平均。然后图像存储器数据可以被过滤并显示在视频监视器上，或者直接通过通用接口总线（GPIB）发送到控制器PC，在PC监视器上以各种格式显示。图像存储器数据可以保存到磁盘或视频，并且以前存储的数据可以从磁盘重新加载到系统上。