Analyzer or photo-emitting biomaterials

分析仪或光发射生物材料

• 批准号: 03557105
• 负责人: KOYAMA Tomiyasu
• 金额: $ 4.42万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03557105/
• 关键词: Photo-emitting biomaterials; Molecular oscillation; Phospholipids; Phosphorous headportion; Glycerol skeleton; Phase transition; Hexagonal II phase; Langmuir-Blodget membrane; LB膜; 傾斜角; 揺動角; 拡散速度; 脂質二重層; ヘキサゴナルII相(HII); ジアシルグリセロール; 回転緩和時間 /

A new system for analyzing photo-emitting biomaterials was constructed with a sensitive photodetector, optic devices and mode-locked pulsed laser. Applications of this system were made for the analysis of the dynamic microstructure of suspended and fixed materials.1. Fluorescent particles accumulating in cardiac tissues of aged rats were collected from ventricular tissues and suspended in salt solution. The obtained results suggested that the size of fluorescent component was only several hundred Daltons and oscillated in a wide range.2. Suspension studies on liposomes, which are experimental models for biomembranes, showed that the head groups and the glycerol skeletons of phospholipids oscillated with a rotational diffusion constant of the order of 10^<-7>/sec.3. Interesting results were obtained on the transition of bilayr to hexagonal phases of phosphlipid layrs. The phase transition induced by incubation of lipisomes with diacylglycerol produced a decrease in head group oscillation and an increase in fatty acyl chain movement, indicating curvature changes of lipid layrs.4. A new idea of fractioned polarization was proposed for the analysis of the polarized light emitted from fixed materials. With the aids of this idea, the main angle of obliquely standing phospholipid molecules, wobbling angle around the main angle and rotational diffusion constant could be calculated from the polarized fluorescent light emitted from fluorophore-labeled Langmuir-Blodget lipid membranes.

利用高灵敏度的光电探测器、光学器件和锁模脉冲激光器，建立了一种新型的生物材料发光分析系统。该系统应用于悬浮和固定材料的动态微观结构分析.从心室组织中收集聚集在老年大鼠心脏组织中的荧光颗粒，并将其悬浮在盐溶液中。结果表明，荧光组分的分子量只有几百道尔顿，且在很宽的范围内振荡.对脂质体（生物膜的实验模型）的悬浮研究表明，磷脂的头部基团和甘油骨架以10^ /秒3量级的旋转扩散常数振荡<-7>。在磷脂层的双层相到六方相的转变上得到了有趣的结果。与甘油二酯孵育诱导的相变导致头基振荡减少和脂肪酰基链运动增加，表明脂质层曲率的变化.提出了一种新的分析固定材料偏振光的方法--分数偏振法。借助这一思想，可以从荧光团标记的Langmuir-Blodget脂膜发出的偏振荧光中计算出倾斜站立磷脂分子的主角度、围绕主角度的摆动角和旋转扩散常数。

项目成果

Mukasa K, Araiso T, Kimura N: "Estimation of molecular motion in the LB film using time-resolved fluorescence depolarization in order to control the film structure." New Functionality Materials. (Elsvier Science Publishers). vol V. 705-710 (1993)

Mukasa K、Araiso T、Kimura N：“利用时间分辨荧光去偏估计 LB 薄膜中的分子运动，以控制薄膜结构。”

荒磯恒久: "膜脂質の流動性と膜タンパク質の動態" 膜. 19. 3-13 (1994)

Tsunehisa Araiso：“膜脂的流动性和膜蛋白的动力学”膜。19. 3-13 (1994)

Kimura N, Tsuneta R, Araiso T, Mukasa K: "Time-resolved fluorescencs depolarization to study the molecular motion of organic thin films." the journal "Langmuir". (submitted to).

Kimura N、Tsuneta R、Araiso T、Mukasa K：“时间分辨荧光去极化研究有机薄膜的分子运动。”

荒磯 恒久: "蛍光偏光法による燐脂質二重層のグリセロール骨格部の運動測定" 第69回日本生理学会大会予稿集. P231 (1992)

Tsunehisa Araiso：“使用荧光偏振测量磷脂双层的甘油骨架的运动”第 69 届日本生理学会年会论文集 P231（1992 年）。

Kimura N,Tsuneta R,Araiso T,Mukasa K: "Time-resolved fluorescence depolarization to study the molecular motion of organic thain films" Submitted to the journal “Langmuir".

Kimura N、Tsuneta R、Araiso T、Mukasa K：“时间分辨荧光去偏振研究有机薄膜的分子运动”，提交给《Langmuir》杂志。