Genetic flux between chloroplast and mitochondria genomes in Triticum and Aegilops.

小麦和山羊草叶绿体和线粒体基因组之间的遗传通量。

• 批准号: 03640542
• 负责人: OGIHARA Yasunari
• 金额: $ 1.28万
• 依托单位: Kihara Institute for Biological Research, Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03640542/
• 关键词: Genetic flux; Organellar genome; Mitochondria DNA; Physical mapping; Chloroplast genes; Triticum and Aegilops; RFLP; Promiscuous DNA; 転写; オルガネラゲノム; 偽遺伝子; 遺伝子発現調節; ゲノム進化

Genetic fluxes among plant organella have been reported in many plants. To investigate aspects of the translocation of genetic material between chloroplast and mitochondrial genomes in the wheat group, we carried out physical mapping of the translocated chloroplast genes on the master copy of mitochondrial DNA of common wheat, and RFLP analysis of mitochondrial DNAs in Triticum and Aegilops by probing translocated chloroplast genes. Specific probes for 20 chloroplast genes were constructed by PCR. Mapping data show that homologous sequences with the nine chloroplast genes, were detected in the mitochondria genome. of nine chloroplast genes, three were transcription activity in the mitochondria as detected by Northern hybridizaition. Hybridization profiles of mitochondrial DNAs from 14 alloplasmic lines of wheat probed with chloroplast atpA and psaA genes revealed high variabilities of RFLP for both genes. Based on the hybridized fragment similarity, phylogenetic relationships were estimated. Different relatedness among 14 plasma types were obtained depending on individual genes.

植物细胞器间的遗传通量在许多植物中都有报道。为了研究小麦群体中叶绿体和线粒体基因组之间遗传物质的易位，我们对易位的叶绿体基因在普通小麦线粒体DNA主拷贝上进行了物理定位，并通过探测易位的叶绿体基因对小麦和山羊草的线粒体DNA进行了RFLP分析。利用PCR技术构建了20个叶绿体基因的特异性探针。定位数据显示，线粒体基因组中检测到与9个叶绿体基因的同源序列。北方杂交结果表明，9个叶绿体基因中有3个在线粒体中有转录活性。以叶绿体atpA和psaA基因为探针，对14个小麦异源细胞质系的线粒体DNA进行杂交分析，发现这两个基因的RFLP变异性很高。基于杂交片段的相似性，系统发育关系进行了估计。根据个体基因的不同，14种血浆类型之间的相关性不同。

项目成果

Y.Ogihara and S.Yanagawa: "Interorganellar translocation of chloroplast genes into mitochondoria." Wheat Information Service. (1993)

Y.Ogihara 和 S.Yanakawa：“叶绿体基因的细胞器间易位到线粒体中。”