Development of polymer medicines production system ulilzing polarized epithelial culturing method.

点击翻译按钮获取中文摘要

Polarized epithelial cells are characterized by two plasma membrane domains, apical and basolateral domains, having different structure and functions. The polarity of epithelial cells is not only restricted to plasma membrane components but secretory proteins are also released in a polarized fashion. MDCK cells are a typical epithelial cell line derived from dog kidney and have been widely used to investigate molecular mechanism of vectorial transport of membrane lipids and proteins. Erythropoietin (Epo) is a major physiological regulator of erythropoiesis by stimulating proliferation and differentiation of erythroid precursor cells. We here found that recombinant human Epo is preferentially secreted from the apical domain of MDCK cells. Taking advantage of this polarized secretion, we have developed a pilot system of producing Epo in industrial scale. We have collected basic information of deigning hollow-fiber system in which Epo will be released either inside or outside of the fiber.