Development of an imaging system for genome mapping

开发基因组图谱成像系统

• 批准号: 04557119
• 负责人: YOSHIDA Michihiro
• 金额: $ 9.92万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04557119/
• 关键词: Genome; Mapping; Image analyzer; FISH; Chromosomes; In situ hybridization; プローブ; 画像処理

Development of an imaging system for chromosome analysis, particularly fluorescence in situ hybridization (FISH), is important for the human genome mapping. This research project aimed for development of an easy-and-cheap digital imaging processor for chromosome mapping :(1) For capture FISH-metaphases as digital imaging we improved a CCD camera and software.(2) Using the improved camera, we also developed and improved software for multicolor FISH-mapping.Our improvements of digital imaging processors by this project have provided more rapid analysis of FISH-signals and chromosomes. However, it is necessary to do more improvement for image enhancements for clearer image and remove background distortions.

染色体成像系统的发展，特别是荧光原位杂交（FISH），是人类基因组定位的重要组成部分。本研究旨在开发一种简单、廉价的染色体定位数字图像处理器：（1）改进了CCD摄像机和软件，实现了FISH中期细胞的数字成像。(2)利用改进后的照相机，我们还开发和改进了FISH作图软件，通过本项目对数字图像处理器的改进，提供了更快速的FISH信号和染色体分析。然而，为了使图像更清晰，去除背景失真，还需要对图像进行进一步的增强。

项目成果

Yoshida,M.C.: "Recent advances in fluorescence in situ hybridization" J.Radiat.Res.Suppl.33. 54-60 (1992)

Yoshida,M.C.：“荧光原位杂交的最新进展”J.Radiat.Res.Suppl.33。

吉田 廸弘: "臨床遺伝学III(古庄,外村,清水,北川編)" 診断と治療社, 643 (1992)

Takahiro Yoshida：“临床遗传学 III（古商、殿村、清水和北川编辑）”诊断与治疗出版，643（1992）

Asano,H.,等: "The mouse Mel-18 “RING-finger" gene:genomic organization,promoter analysis and chromosomal assignment" DNA Sequence. 3. 369-377 (1993)

Asano, H., et al.：“小鼠 Mel-18“RING-finger”基因：基因组组织、启动子分析和染色体分配”DNA 序列 3. 369-377 (1993)。

Ono T, Oshida T & Yoshida MC: "In situ hybridization with radioisotope." Tissue Cult.19(1). 3-7 (1993)

小野T、押田T

Asano H, Ishida A, Hasegawa M, Ono T, Yoshida MC, Taniguchi M & Kanno M: "The mouse Mel-18 RING-finger gene : genomic organization, promotor analysis and chromosomal assignment." DNA Sequence. 3. 369-377 (1993)

浅野 H、石田 A、长谷川 M、小野 T、吉田 MC、谷口 M