The Paragonimus westermani Group in India -Systematics and Genetic Variation

印度卫氏并殖吸虫群 - 系统学和遗传变异

• 批准号: 05041116
• 负责人: KAWASHIMA Kenjiro
• 金额: $ 2.24万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05041116/
• 关键词: Paragonimus; Systematics; Genetic Variation; Bionomics; Host-specificity; Karyotype Analysis; Isozyme Analysis; India; Asia

The present study is to make clear what Paragonimus westermani is which was described by Kerbert in 1878 as a new species, Distoma Westermani on the basis of the specimens from a Bengal tiger, together with the systematic and genetic relations as well as their life histories of Paragonimus species occurring in some endemic areas of paragonimiasis, north-eastern India.During the period of September 23, 1993 - October 16, 1993, the field investigation was carried out in Manipur, north-eastern India. A total of 894 freshwater crabs belonging to two species ; 678 Potamiscus manipurensis and 216 Barytelphusa lugbris were collected from 12 localities of 7 areas in Manipur and examined for Paragonimus infection. Eight hundred and twenty-seven Paragonimus metaceracriae were isolated from P. manipurensis and one was from Barytelphusa lugbris. The former species was proved to be an important crab host and source of human Paragonimus infection in this area. The metacercariae obtained were provisionally classified into 4 groups : "Large P. westermani type", "Small P. westermani type", "Excysted type" and "P. heterotremus type". The number of respective type of Paragonimus metacercariae obtained was as follows : 401, 31, 383 and 13. They were inoculated into dogs, cats and rats, respectively.The morphology of adult flukes, originating from the metacercariae of Large and Small P. westermani types was in good agreement with the description of P. westermani by Vevers(1923), but not by Kerbert(1878).Further systematic, genetic and molecular biological studies on Indian Paragonimus are now in progress.

本研究旨在弄清Kerbert于1878年根据孟加拉虎标本描述的新种Distoma Westermani是什么，以及印度东北部并殖吸虫病流行地区出现的并殖吸虫属物种的系统和遗传关系及其生活史。在1993年9月23日至10月16日期间， 1993年，实地考察 是在印度东北部的曼尼普尔邦进行的。淡水蟹共894只，分属两个品种；从曼尼普尔邦7个地区的12个地点采集了678只马尼普尔鼠和216只红鳝，检查了并殖吸虫感染情况。从 P. manipurensis 中分离出 827 条并殖吸虫，其中 1 条来自 Barytelphusa lugbris。前种被证明是该地区重要的螃蟹宿主和人类并殖吸虫感染源。将获得的囊蚴暂时分为4组：“大韦氏囊蚴型”、“小韦氏囊蚴型”、“囊蚴型”和“异震囊蚴型”。获得的各类型卫氏并殖吸虫囊蚴数量分别为：401、31、383和13个，分别接种到狗、猫和大鼠体内。来自大、小韦氏并殖吸虫型囊蚴的成虫形态与Vevers(1923)对韦氏并殖吸虫的描述非常吻合，但与Vevers(1923)对韦氏并殖吸虫的描述不符。 Kerbert(1878)。对印度并殖吸虫的进一步系统、遗传和分子生物学研究正在进行中。

项目成果

Shibahara,T., Aohagi,Y., Terasaki,K., Koga,M., Kawashima,K., Singh,T.S., Singh,M.S. and Singh,Y.I.: "Morphological features of four types of Paragonimus metacercariae from Manipur, India and the experimental infection of some mammals" Jpn.J.Parasitol.43.

Shibahara,T.、Aohagi,Y.、Terasaki,K.、Koga,M.、Kawashima,K.、Singh,T.S.、Singh,M.S.

Habe,S.,Lai,K.P.F.,Agatsuma,T.,Ow-Yang,C.K. & Kawashima,K.: "Crab hosts for Paragonimus westermani (Kerbert,1878) in Malaysia" Japanese Journal of Tropical Medicine & Hygiene. 21. 137-142 (1933)

Habe,S.,赖K.P.F.,吾妻T.,欧阳,C.K.

柴原壽行、青萩芳幸、寺崎邦生、古賀正崇、川島健治郎、T.S.Singh,M.S.Singh,Y.I.Singh: "インド・マニプール州産肺吸虫メタセルカリア4型の形態的特徴と数種実験動物への感染実験" 寄生虫学雑誌. 43. 139 (1994)

Toshiyuki Shibahara、Yoshiyuki Aohagi、Kunio Terasaki、Masataka Koga、Kenjiro Kawashima、T.S.Singh、M.S.Singh、Y.I.Singh：“印度曼尼普尔邦 4 型肺吸虫囊蚴的形态特征以及几种实验动物的感染实验。”寄生虫学。43。139（1994）

Agatsuma,T.,Lai,P.K.F.,Ow-Yang,C.K.,Habe,S.,Sugiyam,H.,Hirai,H.& Kawashima,K.: "Genetic differentiation between Malaysian and other Asian Paragonimus westermani" Tropical Biomedicine. 10. 45-52 (1933)

吾妻T.，赖P.K.F.，欧阳，C.K.，Habe，S.，杉山H.，平井H.

Habe,S., Lai,K.P.F., Agatsuma,T., Ow-Yang,C.K. and Kawashima,K.: "Crab hosts for Paragonimus westermani (Kerbert, 1878) in Malaysia" Jpn.J.Trop.Med.Hyg.21. 137-142 (1993)

Habe,S., 赖,K.P.F., 吾妻,T., 欧阳,C.K.