On the Regulation of Membrane Biogenesis by Using Yeast Model Systems

点击翻译按钮获取中文摘要

We have a working hypothesis on the ER membrane biogenesis ; that is, when one of many protein components of the ER membrane is produced in a large amount, the protein is incorporated into the ER membrane to disturb it in some way, then unknown signals are produced from the membrane, which induce synthesis of various components of the membrane. Our idea is that this is one of the mechanisms to proliferate cellular membrane in general by stimuli from outside the cells.To prove this, we constructed an expression vector for a yeast, Candida maltosa, which is known to proliferate the ER membrane under some specific culture conditions. By using this expression vector, we have induced cytochrome P450, one of the major ER membrane protein components of this yeast and examined proliferation of the ER membrane by immuno-electron microscopy. We have found that the result of this experiment support our working idea on the ER membrane proliferation as described above.