On the Regulation of Membrane Biogenesis by Using Yeast Model Systems

利用酵母模型系统调控膜生物发生

• 批准号: 05044123
• 负责人: TAKAGI Masamichi
• 金额: $ 1.6万
• 依托单位: Faculty of Agriculture, The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05044123/
• 关键词: yeast; Candida maltosa; ER membrane; cytochrome P450; expression vector; Immuno-electron microscopy

We have a working hypothesis on the ER membrane biogenesis ; that is, when one of many protein components of the ER membrane is produced in a large amount, the protein is incorporated into the ER membrane to disturb it in some way, then unknown signals are produced from the membrane, which induce synthesis of various components of the membrane. Our idea is that this is one of the mechanisms to proliferate cellular membrane in general by stimuli from outside the cells.To prove this, we constructed an expression vector for a yeast, Candida maltosa, which is known to proliferate the ER membrane under some specific culture conditions. By using this expression vector, we have induced cytochrome P450, one of the major ER membrane protein components of this yeast and examined proliferation of the ER membrane by immuno-electron microscopy. We have found that the result of this experiment support our working idea on the ER membrane proliferation as described above.

我们对内质网膜的生物发生有一个可行的假设；即当内质网膜的多种蛋白质成分中的一种大量产生时，该蛋白质被掺入内质网膜，以某种方式干扰内质网膜，然后从膜上产生未知信号，诱导膜的各种成分合成。我们的想法是，这是细胞膜在细胞外部刺激下增殖的机制之一。为了证明这一点，我们构建了酵母的表达载体，念珠菌麦芽糖，已知在某些特定的培养条件下增殖内质网膜。利用该表达载体诱导了该酵母内质膜蛋白主要成分之一的细胞色素P450，并通过免疫电镜观察了内质膜的增殖情况。我们发现这个实验的结果支持了我们关于内质网膜增殖的工作思路。