A study for gene expression of synthetic enzymes and receptors for lipid biofactors

本项目主要研究前列腺素、血小板活化因子、类固醇激素、维生素A和维生素D等生物活性脂类调节剂合成过程中限速酶的分子结构和功能，以及生物活性中受体/信号转导系统。12-脂氧合酶的cDNA克隆及其表达和纯化，细胞分裂素或β-胡萝卜素对前列腺素H合酶-2和12-脂氧合酶的诱导（Yamamoto著）。PAF乙酰水解酶的cDNA克隆和表达，以及编码Miller-Dieker无脑畸形的酶的α亚基的鉴定（由Inoue）。PAF（Shimizu）、5种前列腺素基本受体（DP、EP、FP、IP、TP）、4种EP亚型和3种EP亚型（Narumiya & Ichikawa）的cDNA克隆和组织分布及其基因表达和信号转导。(2)类固醇激素组;类固醇11 β/18-羟基化和醛固酮产生的调节，以及三种CYP 11B基因的分离和表征，包括11 β-羟化酶、醛固酮合成酶和一种新基因，CYP 11B基因启动子区域的分析（Ishimura & Okamoto）。Ad 4 BP的表达和组织特异性定位，一种类固醇生成细胞特异性转录因子（Morohashi）。(3)维生素A和D组;视黄酸反应基因产物、中期因子、各种组织的生长/分化因子的分离和表征、表达和组织分布（Muramatsu）。1 α，25-二羟维生素D3-24-羟化酶5 '侧翼区的mRNA表达调控和维生素D反应元件的鉴定（苏达著）

The project has intended to research in molecular structures and functions of rate-limiting enzymes in the synthesis, and receptors/signal transduction systems in the biological activities for various bioactive lipid modulators ; prostaglandins, PAF,steroid hormones, and vitamin A and D.Summary obtained by research groups as below ;(1) Prostaglandins and PAF group ; cDNA cloning and its expression & purification of 12-lipoxygenase, Induction of prostaglandin H synthase-2 and 12-lipoxygenase by cytokinines or prostagalndins (by Yamamoto) . cDNA cloning and expression of PAF acetylhydrolase, and identification of its alpha subunit of enzyme encoding Miller-Dieker lissencephaly (by Inoue) . cDNA cloning and tissue distribution, and its gene expression and signal transduction of PAF (by Shimizu) , and of 5 basic prostaglandin receptor types (DP,EP,FP,IP,TP) , 4EP subtypes and 3 EPs isoforms (by Narumiya & Ichikawa) . (2) Steroid hormone group ; Regulation of steroid 11beta/18-hydroxylation and aldosterone production, and Isolation and characterization of three CYP11B genes, comprising 11beta-hydroxylase, aldosterone synthetase and a novel genes, analysis of promoter regions of CYP11B genes (by Ishimura & Okamoto) . Expression and tissue specific localization of Ad4BP,a steroidgenic cell-specific transcription factor (by Morohashi) . (3) Vitamins A & D group ; Isolation and characterization, expression and tissue distribution of retinoic acid responsive gene product, midkine, growth/differentiation factor of various tissues (by Muramatsu) . Regulation of mRNA expression, and Identification of vitamin D responsive element in the 5'-flanking region of 1alpha, 25-dihydroxyvitamin D3-24-hydroxylase (by Suda)