PREVENTION OF HYPERACUTE XENO GRAFT REJECTION BY REDUCTION OF NATURAL ANTIBODIES USING DOUBLE FILRATION PLASMAPHERESIS WITH EXVIVO XENOANTIBODY ABSORPTION AND BY INTRAVENOUS POLYCLONAL ANTIBODY AGAINST DONOR BLOOD CELLS INJECTION.

通过使用体外异抗体吸收的双过滤血浆置换法减少天然抗体以及静脉注射抗供体血细胞的多克隆抗体来预防超急性异种移植物排斥。

• 批准号: 05404045
• 负责人: AGASHI Tetuzou
• 金额: $ 8.26万
• 依托单位: Tokyo Wemen's Medical college, Kidney Center
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05404045/
• 关键词: HYPERACUTE REJECTION; DISCORDANT XENOGRAFT; NATURAL ANTIBODY; DOUBLE FILTRATION PLASMAPHERESIS; XENOANTIBODY ABSORPTION; POLYCLONAL ANTIBODY; 拒絶反応; 異種移植; 異種ポリクロナール抗体; 異種自然抗体; 血清補体価; 二重濾過血漿交換; 体外臓器灌流; リンパ球毒性抗体; 赤血球凝集抗体

In 1993, to prevent hyperacute rejection in discordant xenotransplantation, the double filtration plasmapheresis (DFPP) technique and ex vivo xenoantibody absorption were applied to the removal of the natural xenoantibodies. Swine hearts were heterotopically transplanted in the canine necks after the combined DFPP and ex vivo splenic absorption. The mean graft survival time treated by the combined DFPP and ex vivo splenic absorption was prolonged to 244<plus-minus>164 minutes. While it was 9<plus-minus>5 minutes without any treatment. DFPP with ex vivo splenic absorption effectively removed the natural antibodies and resulted in hte prolongation of xenograft surviFrom 1994 to 1995, immunizing rabbits against guinea pig lymphocytes and red cells, we produced special polyclonal antibodies (pAb) and began to investigate the possibulity of preventing hyperacute rejection of guinea pig hearts grafted to rats by the intravenous infusion of pAb. Survival time of vascualized cardiac xenografts was extended from mean of 12 minutes to more than 7 hours, and was closely related to doses of rabbit pAd (r=0.958). Our data suggested that rabbit pAb infusion at high dose might interfere with the reaction of xenoreactive natural antibodies and modulate the complement activation.

1993年，为了预防异种移植中的超急性排斥反应，应用双重过滤血浆置换（DFPP）技术和体外异种抗体吸附来去除天然异种抗体。采用DFPP和离体脾吸收相结合的方法，将猪心异位移植于犬颈部。DFPP和离体脾吸收联合治疗的平均移植物存活时间延长至244 - <plus-minus>164分钟。而不做<plus-minus>任何处理的时间为9.5分钟。1994年至1995年，我们用豚鼠淋巴细胞和红细胞免疫家兔，制备了特异性多克隆抗体（pAb），并开始研究静脉滴注pAb预防豚鼠心脏移植超急性排斥反应的可能性。兔心移植物存活时间由平均12分钟延长至7小时以上，并与pAd剂量密切相关（r=0.958）。结果提示，大剂量兔pAb输注可能干扰异种反应性天然抗体的反应，调节补体激活。

项目成果

H.LIU: "Removal of Immunoglobulins by Exchange Blood Transfusion Utilizing PHP Solution" Therapeutic Plasmapheresis XII. 389-391 (1993)

H.LIU：“利用 PHP 溶液通过换血去除免疫球蛋白”治疗性血浆置换 XII。

劉輝: "異種心臓移植における自然抗体除去に対する安定化ヘモグロビン溶液を用いた全血置換の効果" 人工臓器. 22(3). 993-998 (1993)

刘辉：“使用稳定血红蛋白溶液进行全血置换对异种心脏移植中天然抗体去除的影响”人工器官22(3)。

M. SHINKAI: "Prolongation of cardiac Xenograft Survival by Double Fitration Plasmapheresis and ex vivo Xenoantibody Adsorption" Therapeutic Plasmapheresis. XII. 357-361 (1993)

M. Shinkai：“通过双重过滤血浆置换和离体异种抗体吸附延长心脏异种移植物的存活”治疗性血浆置换。

M. SHINKAI: "Prolongation of cardiac Xenograft Survival by Double Filtration Plasmapheresis and ex vivo Xenoantibody Adsorption" Therapeutic Plasmapheresis. XII. 357-361 (1993)

M. Shinkai：“通过双重过滤血浆置换和离体异种抗体吸附延长心脏异种移植物的存活”治疗性血浆置换。

H. LIU: "Removal of Immunoglobulins Exchange Blood Transfusion Utilizing PHP Solution" Therapeutic Plasmapheresis. XII. 389-391 (1993)

H. LIU：“利用 PHP 溶液去除免疫球蛋白交换输血”治疗性血浆置换术。