Photodamage repair of DNA by anti thymine dimer antibody
Photodamage repair of DNA by anti thymine dimer antibody
批准号:
05404073
负责人:
OHTSUKA Eiko
金额:
$16.13万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (A)
财政年份:
1993
资助国家:
日本
项目状态:
已结题
起止时间:
1993 至 1994
中文摘要
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英文摘要
Two types of cDNAs encoding mouse immauoglobulin heavy (VH) and light (Vc) chain variable (V) domains against UV-induced DNA damage have been prepared. The cDNA was cloned for seyuencing and expression, and the nucleoticle sequences of the V domains were determined by PCR.Genes for a linker (Gly_4-Ser)_3 was inserted between the two domains, and the gene was expressed using the T7 promoter.The single chain Fv for pyrimidine (6-4) photo product was purified and tested for its ability to oligonucleotides containing the (6-4) photoproduct. The scFv was found to be specific.
期刊论文(3)
专著(0)
科研奖励(0)
会议论文
森岡 弘志: "Cloning of V_H and V_L genes for antibodies specific for pyrimidine photo dimers" Nucleic Acids Symposium Series. 29. 111-112 (1993)
Hiroshi Morioka:“嘧啶光二聚体特异性抗体的 V_H 和 V_L 基因的克隆”核酸研讨会系列 29. 111-112 (1993)。
DOI:
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发表时间:
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影响因子:
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作者:
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通讯作者:
MORIOKA,Hiroshi: "Cloning of V_H and V_L genes for antibodies specific for pyrimidine photo dimers" Nucleic Acids Symposium Series. 29. 111-112 (1993)
MORIOKA,Hiroshi:“克隆 V_H 和 V_L 基因以获得嘧啶光二聚体特异性抗体”核酸研讨会系列。
DOI:
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发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Hiroshi Morioka: "Cloning of V_H and V_L genes for antibodies specific for pyrimidine photo dimers" Nucleic Acids Symposium Series. 29. 111-112 (1993)
Hiroshi Morioka:“克隆 V_H 和 V_L 基因以获得嘧啶光二聚体特异性抗体”核酸研讨会系列。
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Elucidation of recognition mechanisms of antibodies specific for nucleic aicd derivatives
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批准号:08101001
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项目类别:Grant-in-Aid for Specially Promoted Research
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资助金额:$186.88万
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财政年份:1996
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负责人:OHTSUKA Eiko
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依托单位:
Antisense Activities of Ribozymes in in vivo
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批准号:03404061
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项目类别:Grant-in-Aid for General Scientific Research (A)
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资助金额:$14.78万
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财政年份:1991
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负责人:OHTSUKA Eiko
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依托单位:
Elucidation of the Mechanism for the Self-Cleavage Rection of RNA Using Chemically-Synthesized Oligonucleotides
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批准号:01480489
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$4.1万
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财政年份:1989
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负责人:OHTSUKA Eiko
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依托单位:
Development of site-specific RNA cleaving method using modified DNA splints-RNase H system
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批准号:62870091
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项目类别:Grant-in-Aid for Developmental Scientific Research
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资助金额:$3.33万
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财政年份:1987
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负责人:OHTSUKA Eiko
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依托单位:
Development of cancer diagnosis using monoclonal antibodies specific for ras gene products
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批准号:60870082
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项目类别:Grant-in-Aid for Developmental Scientific Research
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资助金额:$3.84万
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财政年份:1985
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负责人:OHTSUKA Eiko
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依托单位:
Stereospecific synthesis of phosphotriesters by using new condensing reagents
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批准号:59570894
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项目类别:Grant-in-Aid for General Scientific Research (C)
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资助金额:$1.22万
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财政年份:1984
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负责人:OHTSUKA Eiko
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依托单位: