课题基金 / 基金详情

Photodamage repair of DNA by anti thymine dimer antibody

Photodamage repair of DNA by anti thymine dimer antibody
抗胸腺嘧啶二聚体抗体对 DNA 的光损伤修复
批准号:
05404073
负责人:
OHTSUKA Eiko
金额:
$16.13万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (A)
财政年份:
1993
资助国家:
日本
项目状态:
已结题
起止时间:
1993 至 1994

项目摘要

项目成果

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中文摘要
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英文摘要
Two types of cDNAs encoding mouse immauoglobulin heavy (VH) and light (Vc) chain variable (V) domains against UV-induced DNA damage have been prepared. The cDNA was cloned for seyuencing and expression, and the nucleoticle sequences of the V domains were determined by PCR.Genes for a linker (Gly_4-Ser)_3 was inserted between the two domains, and the gene was expressed using the T7 promoter.The single chain Fv for pyrimidine (6-4) photo product was purified and tested for its ability to oligonucleotides containing the (6-4) photoproduct. The scFv was found to be specific.
期刊论文(3)
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会议论文
森岡 弘志: "Cloning of V_H and V_L genes for antibodies specific for pyrimidine photo dimers" Nucleic Acids Symposium Series. 29. 111-112 (1993)
Hiroshi Morioka:“嘧啶光二聚体特异性抗体的 V_H 和 V_L 基因的克隆”核酸研讨会系列 29. 111-112 (1993)。
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
MORIOKA,Hiroshi: "Cloning of V_H and V_L genes for antibodies specific for pyrimidine photo dimers" Nucleic Acids Symposium Series. 29. 111-112 (1993)
MORIOKA,Hiroshi:“克隆 V_H 和 V_L 基因以获得嘧啶光二聚体特异性抗体”核酸研讨会系列。
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
Hiroshi Morioka: "Cloning of V_H and V_L genes for antibodies specific for pyrimidine photo dimers" Nucleic Acids Symposium Series. 29. 111-112 (1993)
Hiroshi Morioka:“克隆 V_H 和 V_L 基因以获得嘧啶光二聚体特异性抗体”核酸研讨会系列。
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
Elucidation of recognition mechanisms of antibodies specific for nucleic aicd derivatives
  • 批准号:
    08101001
  • 项目类别:
    Grant-in-Aid for Specially Promoted Research
  • 资助金额:
    $186.88万
  • 财政年份:
    1996
  • 负责人:
    OHTSUKA Eiko
  • 依托单位:
Antisense Activities of Ribozymes in in vivo
Elucidation of the Mechanism for the Self-Cleavage Rection of RNA Using Chemically-Synthesized Oligonucleotides
  • 批准号:
    01480489
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
  • 资助金额:
    $4.1万
  • 财政年份:
    1989
  • 负责人:
    OHTSUKA Eiko
  • 依托单位:
Development of site-specific RNA cleaving method using modified DNA splints-RNase H system
  • 批准号:
    62870091
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research
  • 资助金额:
    $3.33万
  • 财政年份:
    1987
  • 负责人:
    OHTSUKA Eiko
  • 依托单位: