Photodamage repair of DNA by anti thymine dimer antibody

抗胸腺嘧啶二聚体抗体对 DNA 的光损伤修复

• 批准号: 05404073
• 负责人: OHTSUKA Eiko
• 金额: $ 16.13万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05404073/
• 关键词: UV-damaged DNA; Thymine Dimer; PCR Cloning; Single Chain Antibody; Flexible Linker

Two types of cDNAs encoding mouse immauoglobulin heavy (VH) and light (Vc) chain variable (V) domains against UV-induced DNA damage have been prepared. The cDNA was cloned for seyuencing and expression, and the nucleoticle sequences of the V domains were determined by PCR.Genes for a linker (Gly_4-Ser)_3 was inserted between the two domains, and the gene was expressed using the T7 promoter.The single chain Fv for pyrimidine (6-4) photo product was purified and tested for its ability to oligonucleotides containing the (6-4) photoproduct. The scFv was found to be specific.

制备了两种编码小鼠免疫球蛋白重链(VH)和轻链可变区(V)的cDNA，以对抗紫外线诱导的DNA损伤。克隆该基因进行测序和表达，并测定V区核苷酸序列。在V区和V区之间插入连接物(Gly_4-Ser)_3基因，利用T7启动子表达该基因。纯化嘧啶(6-4)光产物的单链抗体，检测其与含有(6-4)光产物的寡核苷酸的结合能力。单链抗体被发现具有特异性。

项目成果

森岡 弘志: "Cloning of V_H and V_L genes for antibodies specific for pyrimidine photo dimers" Nucleic Acids Symposium Series. 29. 111-112 (1993)

Hiroshi Morioka：“嘧啶光二聚体特异性抗体的 V_H 和 V_L 基因的克隆”核酸研讨会系列 29. 111-112 (1993)。

MORIOKA,Hiroshi: "Cloning of V_H and V_L genes for antibodies specific for pyrimidine photo dimers" Nucleic Acids Symposium Series. 29. 111-112 (1993)

MORIOKA,Hiroshi：“克隆 V_H 和 V_L 基因以获得嘧啶光二聚体特异性抗体”核酸研讨会系列。

Hiroshi Morioka: "Cloning of V_H and V_L genes for antibodies specific for pyrimidine photo dimers" Nucleic Acids Symposium Series. 29. 111-112 (1993)

Hiroshi Morioka：“克隆 V_H 和 V_L 基因以获得嘧啶光二聚体特异性抗体”核酸研讨会系列。