還元的TCAサイクルの鍵反応に関与する酸化還元因子

参与还原 TCA 循环关键反应的氧化还原因子

• 批准号: 05760060
• 负责人: 石井 正治
• 金额: $ 0.58万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05760060/
• 关键词: フェレドキシン; 還元的TCAサイクル

好熱・絶対独立栄養性水素細菌 Hydrogenobacter thermophilus の無細胞抽出液からフェレドキシンを単離し、同菌の炭酸固定経路(還元的TCAサイクル)に同物質が関与しているか否かを明らかにすることを目的とした。本研究過程で以下の点が明らかにされた。1 フェレドキシンのアッセイ法の改良当初は同菌由来のヒドロゲナーゼを用いることで実験系を組んでいたが、同酵素はフェレドキシンとの反応効率が低いことが示されたため、alpha-ケトグルタレートオキシドレダクターゼ反応を利用し、メトロニダゾール還元活性の回復という方法でアッセイを行うこととした。2 フェレドキシンの単離上記アッセイ法を用いて無細胞抽出液を各種カラムクロマトに供した所、好気的条件下ではフェレドキシンの失活が激しいことが示された。そこで、嫌気的にカラムクロマトを行った。無細胞抽出液を Q-Sepharose,DEAE5PW,Gel filtrationの各カラムクロマトに供し、クロマト的に単一な標品を得た。3 フェレドキシンの性質精製フェレドキシンは、精製alpha-ケトグルタレートオキシドレダクターゼと反応することが示され、還元的TCAサイクルへの還元力供給物質がフェレドキシンであることが示唆された。

The cell extract was isolated from the bacteria Hydrogenobacter thermophilus, and the same bacteria were fixed with carbonic acid. The same substance was isolated from the same bacteria. In the course of this study, we will discuss the following points. 1. In the first place, the same bacteria were isolated from the same bacteria. In the first place, the same bacteria were isolated from the same bacteria. In the first place, the same bacteria were isolated from the same bacteria. In the first place, the same bacteria were used to make use of the same bacteria. The homozygous enzyme was used in the system, and the reaction rate was lower than that in the control group. The rate was lower than that in the control group, and the rate was low. The results showed that the bacteria were not used in the first place, and that they were used in the same way. The results showed that they were not used. Please do not know how to do this. Please do not know how to do this. 2. In this method, all kinds of fluids are extracted from the cells and are supplied to the hospital under good conditions. under good conditions, they are activated and activated. I don't know, I don't like it, I don't like it, I don't like it. QmurSepharose extract, QmurSepharose, DEAE5PW, Gel filtration, Gel Sepharose extract, cell extract and cell extract. 3. You need to know that you need to know that you have a problem, that is, you know, alpha-, you know, you know, you know,