脳血管障害患者における血液レオロジー因子への好中球産生スーパーオキサイドの影響

中性粒细胞产生超氧化物对脑血管病患者血液流变学指标的影响

• 批准号: 05770440
• 负责人: 浜野 均
• 金额: $ 0.51万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05770440/
• 关键词: 脳血管障害; 赤血球凝集能; 活性酸素; スーパーオキサイド; SOD

I.活性酸素の赤血球凝集能への影響とradical scavengerの効果好中球からsuperoxideの産生を誘導するPMAを全血サンプルに添加すると、赤血球膜が活性酸素により傷害され赤血球凝集能が悪化することは既に報告している。また、in vitroでSOD、ソルコセリルを前添加した後の赤血球凝集能の悪化は、抑制された。さらに、今回は脳梗塞患者にソルコセリル点滴静注、alpha-トコフェロール経口投与を行い、その前後での全血サンプルにPMAを添加し、赤血球凝集能を測定した。赤血球凝集能は、光透過法による自家製aggregometerにてその半値時間を測定し、その指標とした。その結果、(1)5例の脳梗塞患者にソルコセリル2Aを1時間で点滴静注した前後でのPMA添加赤血球凝集能には、推計学的に有意な変化は認められなかった。(2)4例の脳梗塞患者にニコチン酸トコフェロール600mg/日、14日間投与前後でのPMA添加赤血球凝集能にも、推計学的に有意な変化は認められなかった。今回までの結果では、症例数も少なく、今後は症例を増やすこと、また薬剤の投与量、投与期間の再検討も望まれる。II.活性酸素の赤血球変形能に対する影響5例の正常者の血液をサンプルに、in vitroでPMAを添加し、Reidらの変法で赤血球通過時間を測定したところ、有意に延長した。

i. Active acid hemagglutination can affect radical scavenger hemagglutination. If superoxide is good, PMA whole blood hemagglutination can be added, red blood cell membrane can be damaged by red blood cell agglutination, and red blood cell agglutination can be changed. Red blood cell agglutination can cure and inhibit the formation of red blood cells before the addition of red blood cell agglutination before the addition of drugs, in vitro SOD and other drugs. This time, patients with infarct were treated by intravenous drip, alpha- was injected orally, whole blood was added, and red blood cell agglutination could be determined. The red blood cell agglutination energy test and light transmission method were used to determine the half-time measurement of aggregometer in our own company. The results were as follows: (1) 5 patients with acute cerebral infarction were treated with intravenous drip of PMA before and after intravenous drip at the end of two weeks. (2) in 4 patients with acute myocardial infarction, the addition of red blood cell agglutination energy to 600mg/ on the 14th day and 14th day and the addition of red blood cell agglutination energy on the 14th day and 14th day and the addition of red blood cell agglutination energy on the 14th day and 14th day in 4 patients with acute myocardial infarction. The results of this trial are satisfactory, the number of cases is very low, and the number of cases in the future is very low. In the future, we should pay more attention to the number of patients, pay more attention to the number of patients, and re-check the number of patients during the period. ii. In 5 cases of normal patients, red blood cells were detected by active acid PMA, in vitro red blood cells were added, red blood cells were measured by Reidage assay, and intentionally prolonged.