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象牙質知覚過敏処置後の象牙細管構造の変化-イオン導入法とシュウ酸カリウム溶液の効果-

牙本质过敏治疗后牙本质小管结构的变化 - 离子电渗疗法和草酸钾溶液的效果 -

基本信息

批准号：
05771634
负责人：
三好敏朗
金额：
$ 0.58万
依托单位：
The Nippon Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至无数据
项目状态：
已结题

项目摘要

象牙質知覚過敏症の治療法のうち、イオン導入法またはシュウ酸カリウム溶液の塗布法の施した際の象牙細管内部の微細構造変化を調べる目的で、あえて比較的に象牙細管の開口の大きい幼若永久歯を対象に以下の実験を行った。すなわち、根未完成または根完成菌の幼若永久歯を有するカニクイザル2頭を実験動物として用いた。全身麻酔後、実験動物を手術台に固定し、前歯唇面、臼歯咬合面にそれぞれ2ケの独立した象牙質窩洞(深さ約2mm,直径約2mm)を形成し、窩底部に存在するスメア層の除去を超音波スケーラーにて行った。実験群は〔A群〕2窩洞のうち1つには8%塩化亜鉛液のイオン導入0.3mmA5分、他の1つには生理食塩液の塗布、〔B群〕2窩洞のうち1つには25%シュウ酸カリウム溶液2分間塗布、他の1つには2%フッ化ナトリウム溶液を3分間用、の2群とした。各実験群の窩洞から直径約1mm前後の象牙質試料を取り出し、1.樹脂包理後、厚さ約10〜20mumの切片を作製し、各種染色を施し、応用直下の歯髄と硬組織の光学顕微鏡観察、2.通法に従い脱水、臨界点乾燥を行い、走査型電子顕微鏡(SEM)観察、3.一部の試料はエポン樹脂包理後、ダイヤモンドナイフにて超薄切片を作製し、透過型電子顕微鏡観察を行った。その結果、光顕的観察では、塗布面に一致する歯髄腔の表層には特に変化はみられなかったが、残存窩底象牙質の厚さが比較的菲薄な例では、イオン導入例に若干の象牙芽細胞の変化を生じた。SEM観察では、細管の封鎖や狭窄がシュウ酸カリウム応用例にみられたが、同部象牙質直下の歯髄面における変化は現在標本作製中であり、今後、さらに詳細に関して検索していく予定である。

The treatment of dentin allergy is based on the introduction method, the application method and the fine structure adjustment of ivory tubule. The root is not complete, the root is complete, and the young is permanent. After total anesthesia, the animal was fixed on the operating table, and the anterior teeth and occlusal surfaces were separated by 2 mm of dentin cavity (about 2mm deep and 2mm in diameter). The cavity bottom was removed by ultrasound. In the group A, the lead solution of 8% sodium hydroxide was introduced into 0.3mmA5 minutes, and the physiological food solution was coated in 1 minute. In the group B, the lead solution of 25% sodium hydroxide was coated in 2 minutes, and the lead solution of 2% sodium hydroxide was coated in 3 minutes. In the group B, the lead solution of 2% sodium hydroxide was coated in 3 minutes. Dentine samples before and after the cavity of each group with a diameter of about 1mm were taken. 1. After resin coating, sections with a thickness of about 10 ~ 20mum were prepared. Various stains were applied. Optical microscopy of dental hard tissues directly below was used. 2. Dehydration and critical point drying were carried out by method. SEM was used for observation. 3. Ultrathin sections of one sample were prepared after resin coating. transmission electron microscope As a result, optical observation showed that the coating surface was consistent with the surface layer of the tooth cavity, and the thickness of the residual dentin at the bottom of the tooth cavity was relatively thin. SEM observation shows that in the process of manufacturing, the thickness of the thin tube is narrowed, and the tooth surface of the same part of the dentin is changed. In the future, the details of the test are determined.