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Roles of p53 and ras gene mutations in genesis of oral SCC in India

p53 和 ras 基因突变在印度口腔鳞状细胞癌发生中的作用

基本信息

批准号：
06044072
负责人：
TSUCHIDA Nobuo
金额：
$ 1.98万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044072/
关键词：
oral cancer squamous cell carcionoma p53 gene ras gene HPV PCR SSCP p53 癌抑制遺伝子 ras タバコ

项目摘要

In order to find differences of the genesis of oral squamous cell carcinomas in between India and Japan, 53 oral tumor samples obtained from Government Rajaji Hospital, Madurai were analyzed for p53 and ras mutations and another 50 uterine cervical cancer samples from Anna Cancer Hospital, Kancheepuram for p53 and HPV status. DNA was isolated form these tumors and specific regions were PCR-amplified using primers for p53, ras and HPV.The amplifications were done in the presence of 32PdCTP and the products for p53 and ras were screened for mutations by SSCP analysis. SSCP positive samples for p53 were confirmed and sequenced in Tokyo Medical Dental University by A.K.Munirajan who visitied here thrice for this purpose and to learn other techniques. The results indicated p53 mutations in 11 out of 53 (21%) oral samples. Most of these mutations were transition, which is consistent with the involvement of tabacco-related nitrosoamines in the etiology of oral squamous cell carcinoma. Ras mutations were relatively of high frequency (32%). However the p53 and ras mutations did not overlap in a sample. Moreover, most ras mutations were detected in H-ras gene. Unider conditions which detected HPV in 60-70% of cervical cancers we were unable to demosntrate the presence of HPV in the oral cancers, suggesting no significant role of this verus in genesis of Indian oral squamous cell carcinoma.

为了发现印度和日本口腔鳞状细胞癌发生的差异，对来自马杜赖Rajaji政府医院的53例口腔肿瘤样本进行了p53和ras突变分析，并对来自Kancheepuram安娜癌症医院的另外50例宫颈癌样本进行了p53和HPV状态分析。从这些肿瘤中提取DNA，用p53、ras和HPV特异性引物进行PCR扩增，在32 PdCTP存在下进行扩增，并通过SSCP分析筛选p53和ras的突变产物。A.K.Munirajan在东京医科齿科大学对p53的SSCP阳性样本进行了确认和测序，他为此目的在这里学习了三次，并学习了其他技术。结果表明，p53突变11/53（21%）的口腔样本。这些突变大多数是过渡突变，这与烟草相关的亚硝胺参与口腔鳞状细胞癌的病因学是一致的。Ras突变频率较高（32%）。然而，p53和ras突变在样品中没有重叠。ras基因突变主要发生在H-ras基因。在60-70%的宫颈癌中检测到HPV的条件下，我们无法证明口腔癌中HPV的存在，这表明该病毒在印度口腔鳞状细胞癌的发生中没有显著作用。