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c-kit Mutant Rats as a Tool for Mast Cell Research-Development and Function

c-kit 突变大鼠作为肥大细胞研究开发和功能的工具

基本信息

批准号：
06044144
负责人：
KATAMURA Yukihiko
金额：
$ 5.44万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Mast cells play an important role for immediate hypersensitivity reaction as an effector cell. Deseases that are induced as a result of immediate hypersensitivity reaction such as allergic rhinitis, infantile asthma and atopic dermatitis are increasing in industrialized countries. To analize the role of mast cells in immediate hyper-sensitivity reaction, mast cell-deficient animals are useful. Since we recently found mast cell-deficient Ws/Ws rats, we attempted to colaborate with mast cell researchers of various counties by using Ws/Ws rats. Since the original Ws/Ws rats are not inbred. we attempted to introduce the Ws mutant gene into certain inbred strains. However, we cound not find any suitable inbred strains, under which genetic background appreciable number of Ws/Ws rats could be raised. Therefore, we cleaned Ws/Ws rats of closed colony into specific pathogen-free (SPF) condition. SPF condition is required to send experimental animals to foreign countires. Since production of such Ws/Ws rats in the SPF condition appeared to be satisfactory, we are now planning to send these Ws/Ws andcontrol +/+ rats to Harvard, MaMaster and Edingurgh Universities. We recognized that it took rather long period to obtain useful experimental animals that can be transported to the foreign countires. Tsujimura visited Edinburgh Univeirsty to learn about an infection experiment with Shistomsoma mansoni. Kitamura visited Harvard University to explain the delay of the production of Ws/Ws rats in SPF condition. Galli, Perdue and Westling (on behalf of Miller) visited Osaka University to learn the characters and the breeding method of Ws/Wsrats. We all to gether discussed future colaboration by using Ws/Ws rats.

肥大细胞作为效应细胞在速发型超敏反应中发挥重要作用。在工业化国家，由于速发型超敏反应而诱发的疾病，如过敏性鼻炎、婴儿哮喘和特应性皮炎正在增加。为了分析肥大细胞在速发型超敏反应中的作用，肥大细胞缺陷动物是有用的。由于我们最近发现肥大细胞缺陷的Ws/Ws大鼠，我们试图与各国肥大细胞研究人员合作，使用Ws/Ws大鼠。由于原始的Ws/Ws大鼠不是近亲繁殖的。我们试图将Ws突变基因导入某些近交系。然而，我们没有找到合适的近交系，在此遗传背景下，可以提高相当数量的Ws/Ws大鼠。因此，我们将封闭群的Ws/Ws大鼠清洁至无特定病原体（SPF）状态。送实验动物到国外需要SPF条件。由于在SPF条件下生产这样的Ws/Ws大鼠似乎是令人满意的，我们现在计划将这些Ws/Ws和对照+/+大鼠送到哈佛、MaMaster和Edingurgh大学。我们认识到，要获得可以运送到国外的有用的实验动物需要相当长的时间。Tsujimura访问了爱丁堡大学，了解曼氏血吸虫的感染实验。北村访问了哈佛大学，解释了SPF条件下Ws/Ws大鼠生产延迟的原因。Galli、Perdue和Westling（代表米勒）访问大坂大学，了解Ws/Wsrats的性状和育种方法。利用Ws/Ws大鼠对今后的合作进行了讨论。