Molecular biological significance of asialoglycoprotein receptor in autoimmune hepatitis type I
自身免疫性I型肝炎脱唾液酸糖蛋白受体的分子生物学意义
基本信息
- 批准号:06454261
- 负责人:
- 金额:$ 4.61万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1994
- 资助国家:日本
- 起止时间:1994 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Reverse transcription and polymerase chain reaction was applied to isolate cDNAs encoding asialoglycoprotein receptor (ASGPR) H1 and L-H2 from human liver mRNAs. Using these cDNAs, recombinant ASGPR protein was expressed in E.coli. Rabbit antisera specific to human ASGPR H1 or L-H2 was obtained by immunizing these recombinant proteins. Enzyme-linked immunosorbent assay (ELISA) using bacterial recombinant antigen was developed to measure serum antibodies to ASGPR.Sera from autoimmune hepatitis, primary biliary cirrhosis, alcoholic liver disease, chronic hepatitis C,chronic hepatitis B,systemic lupus erythematosus, and healthy subjects were tested. High OD value was obtained almost exclusively in autoimmune hepatitis and chronic hepatitis C.The OD value of anti-ASGPR antibodies detected by ELISA using recombinant antigen was correlated with anti-ASGPR antibody titers that were determined by ELISA using human liver antigen. By using extracellular domain of ASGPR protein in ELISA,similar sensitivity to pick-up anti-ASGPR antibodies was demonstrated in autoimmune hepatitis and less reactivity was detected in chronic hepatitis C.It was suggested that main epitope which was reactive with antibodies in autoimmune hepatitis could be located on the extracellular domain of the ASGPR protein. Lymphocytes from peripheral blood mononuclear cells in a patient with autoimmune hepatitis showed a stimulation index as 4 in lymphocyte stimulation test with recombinant ASGPR antigen. There could exist a certain class of lymphocytes reactive with human ASGPR in autoimmune hepatitis patients.
应用逆转录-聚合酶链反应技术从人肝组织中分离编码去唾液酸糖蛋白受体(ASGPR)H1和L-H2的cDNA。利用这些cDNA,重组ASGPR蛋白在大肠杆菌中表达。用重组蛋白免疫兔获得抗人ASGPR H1和L-H2的兔抗血清。应用细菌重组抗原建立酶联免疫吸附试验(ELISA),检测自身免疫性肝炎、原发性胆汁性肝硬化、酒精性肝病、慢性丙型肝炎、慢性B型肝炎、系统性红斑狼疮和健康人血清中抗ASGPR抗体。高OD值几乎完全在自身免疫性肝炎和慢性丙型肝炎中获得。使用重组抗原通过ELISA检测的抗ASGPR抗体的OD值与使用人肝抗原通过ELISA测定的抗ASGPR抗体滴度相关。用ASGPR蛋白胞外区进行ELISA检测,发现自身免疫性肝炎对抗ASGPR抗体的敏感性相似,而慢性丙型肝炎对抗ASGPR抗体的敏感性较低,提示自身免疫性肝炎与抗体反应的主要抗原表位可能位于ASGPR蛋白胞外区。1例自身免疫性肝炎患者外周血单个核细胞经重组ASGPR抗原刺激试验,刺激指数为4。自身免疫性肝炎患者体内可能存在一定种类的ASGPR反应性淋巴细胞。
项目成果
期刊论文数量(31)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
今井,明彦: "自己免疫性肝疾患" 信州医誌. 44. 305-315 (1996)
Imai, Akihiko:“自身免疫性肝病”《信州医学杂志》44. 305-315 (1996)。
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Tsuchiya, K.: "Detection of anti-double and anti-single stranded DNA antibodies in chronic liver disease : significance of anti-double stranded DNA antibody in autoimmune hepatitis" J Gastroenterol. 29. 152-158 (1994)
Tsuchiya, K.:“慢性肝病中抗双链和抗单链 DNA 抗体的检测:抗双链 DNA 抗体在自身免疫性肝炎中的意义”J Gastroenterol。
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一條哲也: "自己免疫性肝疾患-その病態と治療-" 新興医学出版社(in press), (1996)
Tetsuya Ichijo:“自身免疫性肝病 - 其病理学和治疗” Shinko Igaku Publishing(印刷中),(1996)
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- 影响因子:0
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Ichijo T.: "Pathogenesis of autoimmune hepatitis-Current concept-" IGAKU NO AYUMI. 171. 1037-1041 (1994)
Ichijo T.:“自身免疫性肝炎的发病机制-当前概念-” IGAKU NO AYUMI。
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- 影响因子:0
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Ichijo T: "Autoimmune hepatitis type 1 without evidenice of hepatitis G vivus infection" Int.Hepatol.Commun.(in press). (1997)
Ichijo T:“没有证据显示 G 型肝炎病毒感染的 1 型自身免疫性肝炎”Int.Hepatol.Commun.(正在印刷中)。
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{{ truncateString('KIYOSAWA Kendo', 18)}}的其他基金
The role of hepatitis C infection and proliferators-activated receptor alpha protects (PPA α) in occurrence of hepatocellular carcinoma
丙型肝炎感染和增殖物激活受体α保护(PPAα)在肝细胞癌发生中的作用
- 批准号:
15590633 - 财政年份:2003
- 资助金额:
$ 4.61万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Moleculebiological analysis of HLA class II in autoimmune hepatitis.
自身免疫性肝炎HLA II类的分子生物学分析。
- 批准号:
03454227 - 财政年份:1991
- 资助金额:
$ 4.61万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
The Study on anti-PCNA antibody in patient with hepatocellular carcinoma.
肝细胞癌患者抗PCNA抗体的研究。
- 批准号:
61570334 - 财政年份:1986
- 资助金额:
$ 4.61万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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8391632 - 财政年份:2011
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Alcohol Alters Hepatic Immune Function: Role of the Asialoglycoprotein Receptor
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8598019 - 财政年份:2011
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Role of Asialoglycoprotein receptor in tumor microenvironment
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SORTING OF INTESTINAL ASIALOGLYCOPROTEIN RECEPTOR
肠去唾液酸糖蛋白受体的分类
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2135832 - 财政年份:1994
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$ 4.61万 - 项目类别:
SORTING OF INTESTINAL ASIALOGLYCOPROTEIN RECEPTOR
肠去唾液酸糖蛋白受体的分类
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2135833 - 财政年份:1994
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