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Improvement of Long Term Survival for Malignant Ovarian Tumor

提高卵巢恶性肿瘤的长期生存率

基本信息

批准号：
06454470
负责人：
TOMODA Yutaka
金额：
$ 4.54万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06454470/
关键词：
Ovarian Tumor Recurrence MMP Zymography

项目摘要

PVB regimen was superior to CAP regimen for ovarian cancer (p<0.05). Although difference of survival rate increased between 2 regimems within 24 months, thereafter survival rates decreased gradually at the same rate. Furthermore, there were no difference observed between 2 regimen in disease free survival. These results suggested that primary treatment including operation and chemotherapy was very important to improve survival for ovarian cancer. One hundred and fifty borderline malignancy cases were also analyzed. One hundred and twenty-five cases were stage I and 52 cases were managed conservely pregnancy potencial. Although stage I cases received no adjuvant therapy after operation, no cases died, suggesting that adjuvant therapy was meanless in stage I cases. On the other hand, 9 cases died among 25 with stage II or more. Thus, adjuvant chemotherapy including cisplatin might be performed in advanced cases. However, adjuvant chemotherapy would not be effective on mucinous borderline cases as well as mucinous cancer. MMPs and TIMPs have been focused for cancer cell invasion and metastasis. These proteins were examined in gynecologic cancer tissues by using gelatin zymography and reverse zymography. The secretion of MMPs increased more in cancer tissues than that in normal tissues and specially MMP-9 increased remarkably in cancer tissues, although this enzyme was secreted little in normal tissues. The secretion fo TIMP-1 increased more in cancer tissues than that in normal tissues. Furthermore, we examined these proteins in peritoneum, myometrium and ovary. MMP-9 was secreted mostly in peritoneum and TIMP-1 in ovary. Conditioned medium of these normal tissues had the potency to induce MMPs and TIMPs from ovarian cancer cells. These results suggested that the influence of normal tissues on cancer cells was strongly associated with cancer cells invasion.

对于卵巢癌，PVB 方案优于 CAP 方案（p<0.05）。尽管24个月内两种治疗方案之间的生存率差异有所增加，但此后生存率以相同的速度逐渐下降。此外，两种方案之间的无病生存期没有观察到差异。这些结果表明，包括手术和化疗在内的初级治疗对于提高卵巢癌的生存率非常重要。还分析了一百五十个临界恶性肿瘤病例。 125 例处于 I 期，52 例处于保守妊娠状态。尽管Ⅰ期病例术后未接受辅助治疗，但无死亡病例，提示辅助治疗对Ⅰ期病例没有意义。另一方面，25 例 II 期或以上的病例中有 9 例死亡。因此，晚期病例可能需要进行包括顺铂在内的辅助化疗。然而，辅助化疗对粘液交界性病例和粘液癌无效。 MMP 和 TIMP 一直被关注于癌细胞的侵袭和转移。通过使用明胶酶谱和反向酶谱检查妇科癌症组织中的这些蛋白质。癌组织中MMPs的分泌量比正常组织中增多，特别是MMP-9在癌组织中的增加显着，而正常组织中该酶的分泌量很少。癌组织中TIMP-1的分泌量比正常组织中增加更多。此外，我们检查了腹膜、子宫肌层和卵巢中的这些蛋白质。 MMP-9主要在腹膜分泌，TIMP-1主要在卵巢分泌。这些正常组织的条件培养基具有诱导卵巢癌细胞产生 MMP 和 TIMP 的能力。这些结果表明正常组织对癌细胞的影响与癌细胞的侵袭密切相关。