The absorption study with BMIPP in a swine model of partial small intestinal transplantation

本研究旨在建立新型猪小肠移植模型，并利用BMIPP^<**>评价移植物的功能。一期自体肠移植研究在体重10-15kg的小型猪身上进行部分肠自体移植，以阐明手术技术的可行性，并确定肠的最合适部位和长度。证实原位部分回肠移植是可行的。在该模型中，需要超过小肠总长度的30%才能实现长期存活。异体移植采用与1期研究相同的技术进行。免疫抑制方案由FK506 （0.3mg/kg/day）和甲基强的松龙组成。在该模型中，受体存活1个月，移植功能测试成为可能。BMIPP的相吸收研究BMIPP在自体或同种异体部分回肠移植受者中的吸收。结果与对照动物的结果进行了比较。观察了长链脂肪酸吸收功能的可逆性。然而，不同动物吸收功能的恢复情况差异很大，需要进一步研究确定BMIPP在该模型中的临床意义。^<**>: BMIPP,^<123> i -碘苯甲基戊酸酯

This study was designed to establish a new swine model of small intestinal transplantation and to evaluate the graft function using BMIPP^<**>.Phase 1 Autotransplantation studyAutotransplantation of partial intestine was performed in small swine (body weight, 10-15kg) to elucidate the feasibility of the operative technique and to decide the most appropriate part and length of the intestine. Orthotopic partial ileal transplanation was proved to be feasible. More than 30% of the total length of the small intestine was necessary to achieve long-term survival in this model.Phase 2 Allotransplantation studyAllotransplantation was performed in the same technique as in phase 1 study. A immunosuppression protocol consisting of FK506 (0.3mg/kg/day) and methyl prednisolone was employed. One-month survival of the recipients was achieved and the graft function test was possible in this model.Phase Absorption study with BMIPPThe graft absorption of BMIPP was studied in recipients which underwent auto-or allotransplantation of partial ileum. The results were compared with those in control animals. The reversibility of absorptive function of long-chain fatty acids was observed. However, the recovery of absorptive function was so different among animals that further study is needed to decide the clinical significance of BMIPP in this model.^<**> : BMIPP,^<123>I-Iodophenylmethylpentadecanate