The absorption study with BMIPP in a swine model of partial small intestinal transplantation

BMIPP在猪部分小肠移植模型中的吸收研究

• 批准号: 06454504
• 负责人: NIO Masaki
• 金额: $ 4.35万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06454504/
• 关键词: Transplantation; Small intestine; Swine; Absorption; Fat; 生体; 消化吸収; 脂肪; FK506; 拒絶反応; 血管血流量

This study was designed to establish a new swine model of small intestinal transplantation and to evaluate the graft function using BMIPP^<**>.Phase 1 Autotransplantation studyAutotransplantation of partial intestine was performed in small swine (body weight, 10-15kg) to elucidate the feasibility of the operative technique and to decide the most appropriate part and length of the intestine. Orthotopic partial ileal transplanation was proved to be feasible. More than 30% of the total length of the small intestine was necessary to achieve long-term survival in this model.Phase 2 Allotransplantation studyAllotransplantation was performed in the same technique as in phase 1 study. A immunosuppression protocol consisting of FK506 (0.3mg/kg/day) and methyl prednisolone was employed. One-month survival of the recipients was achieved and the graft function test was possible in this model.Phase Absorption study with BMIPPThe graft absorption of BMIPP was studied in recipients which underwent auto-or allotransplantation of partial ileum. The results were compared with those in control animals. The reversibility of absorptive function of long-chain fatty acids was observed. However, the recovery of absorptive function was so different among animals that further study is needed to decide the clinical significance of BMIPP in this model.^<**> : BMIPP,^<123>I-Iodophenylmethylpentadecanate

本研究旨在建立一种新的猪小肠移植模型，并应用BMIPP评价移植物的功能。第一阶段自体小肠移植研究在体重10- 15 kg的小型猪上进行部分小肠的自体移植，以阐明手术技术的可行性，并确定最佳的小肠部分和长度。原位部分回肠移植是可行的。超过30%的小肠总长度是必要的，以实现长期生存在这个model.Phase 2同种异体移植研究同种异体移植是在相同的技术，在第1阶段的研究。采用FK 506（0.3mg/kg/天）和甲基强的松龙组成的免疫抑制方案。BMIPP的阶段吸收研究在接受自体或异体部分回肠移植的受者中研究了BMIPP的移植物吸收。结果与对照组动物进行了比较。观察到长链脂肪酸吸收功能的可逆性。然而，吸收功能的恢复在动物之间是如此不同，需要进一步研究来确定BMIPP在该模型中的临床意义。&lt;**&gt;：BMIPP，<123>对碘苯基甲基十五烷