Surface characterizations of protein adsorbed synthetic hydroxyapatite

一般来说，疏水性/亲水性是通过水润湿的方法来探测的，而水润湿的测量方法则是采用无孔液滴法等方法。然而，当人们试图测量生物大分子被吸附的表面的湿润程度时，由于表面干燥，存在构象变化的问题。因此，这种方法无法获得现场信息。本文提出了一种研究表面蛋白层在水溶液中的润湿性的新方法（滴时法）。研究了蛋白质在水中对羟基磷灰石的吸附行为。进一步研究了表面势能对细菌与羟基磷灰石（HAp）黏附的影响，通过理论计算羟基磷灰石与口腔细菌之间的双电层力（Vel）和范德沃尔斯力（VA）相互作用的表面势能。我们发现，在水瓶座溶液中，吸附在固体表面的蛋白质层的疏水性/亲水性可以用DTM来测量。我们申请了我们开发的DTM专利。此外，我们得出结论，细菌对HAp的粘附可以通过增加pH、降低离子浓度和不加入多价阳离子来抑制。

Generally, the hydrophobicity/hydrophilicity is probed by water wetting, which is measured by a method such as the sessile liquid drop method. However, when one tries to measure the wetting of a surface where biological macromolecules are adsorbed, there is a problem of a change in conformation due to drying the surface. Hence, by this method in situ information can not be obtained. We have developed a new method (Dropping Time Method ; DTM) to investigate the wettability of surface protein layr in aqueous solution. We studied adsorption behavior of proteins on the hydroxyapatite in water. Furthermore We investigated the effect of surface potential energy on the adhesion of bacteria to hydroxyapatite (HAp) using a theoretical calculation of surface potential energy with the interaction of electrical double layr forces (Vel) and van der Walls forces (VA) between HAp and oral bacteria.We found that, the hydrophobicity/hydrophilicity character of the protein layr adsorbed on the surface of solids in aquarius solution can be measured by DTM.We applied a patent of DTM that we developed. Furthermore, we concluded that adhesion of bacteria to HAp can be inhibited by increasing pH,decreasing ion concentration, and not incorporating maltivalent cations in solutions.