Assembling a system for measuring biological activity of micro-level particle in heterogenous system

组装异质系统中微米级颗粒生物活性测量系统

• 批准号: 06554036
• 负责人: YOSHIOKA Takahito
• 金额: $ 3.97万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06554036/
• 关键词: Heterogeneous system; Tracer experiment; YAG laser; Carbon stable Isotope; Mass spectrometer; 微小粒子; 赤外線顕微鏡; 赤外顕微鏡

New system for measuring the biological activity of micro-level particles, for example, planktonic and bacterial cells and their colony, in heterogeneous system was developed. This system, namaed LASMMA (Laser Semi-Microprobe Mass Analysis), is composed of YAG laser, infra-red microscope, quartz cell, gas purification line, and isotope ratio mass spectrometer (IRMS). This system was specialized for the ^<13>C-tracer experiment on phytoplanktonic photosynthesis.Optical system, by which laser beam was applied to the sample through object lens of IR microscope, was prepared. Using TV camera, micro-spot was identified during laser treatment. Cellulose filter, of which isotope ratio was known, was treated with laser for 30 to 60 minutes. After purification and manometric measurement, the CO_2 was diluted with standard CO_2 gas of which ^<13>C content was known. The gas was introduced into IRMS to determine the ^<13>C content. Although the value of cellulose by LASMMA was lower than that by standard method, it suggested that its precision was enough for tracer experiment.LSMMA method was applid in the field experiment. The ^<13>C uptake activity of Microcystis was etsimated in Lake Suwa. Colony of this alga was identified under microscope, and treated by laser. Its ^<13>C content increased from 1.09 atom% at the beginning of the experiment to 3.52 atom% after 24hr incubation. The ^<13>C content changed almost as same as that measured for particulate organic carbon (POC) by the usual way.It was confirmed that LASMMA method could be applicable for field tracer experiment. Moreover, it was suggested that LASMMA system might not be limited for tracer experiment, although the standard deviation measured was larger than usual way and the measurement took much time.For future application, LASMMA System manual was rounded off.

开发了一种用于测量非均质体系中浮游细胞、细菌细胞及其菌落等微观颗粒生物活性的新系统。该系统由YAG激光器、红外显微镜、石英池、气体净化线和同位素比质谱仪组成。该系统是专门用于<13>植物光合作用的~（13）C示踪实验的，其光学系统是将激光束通过红外显微镜的物镜透镜照射到样品上。利用电视摄像机，在激光治疗过程中识别微斑。用激光处理同位素比已知的纤维素过滤器30 ~ 60分钟。CO_2经净化、测压后，用已知~（13）C含量的标准CO_2气体稀释<13>。将气体引入IRMS中以测定13 <13>C含量。虽然LASMMA法测得的纤维素值低于标准法，但其精度足以满足示踪试验的要求，因此，LSMMA法在田间试验中是可行的。对<13>和湖中微囊藻的^ C吸收活性进行了估计。在显微镜下鉴定菌落，并用激光处理。其14 <13>C含量从实验开始时的1.09原子%增加到24小时温育后的3.52原子%。结果<13>表明，LASMMA法测定的颗粒有机碳（POC）含量与常规方法测定的结果基本一致，可用于现场示踪实验。此外，LASMMA系统在示踪剂实验中的应用并不局限于此，尽管其标准偏差比常规方法大，且测量时间较长，但为了今后的应用，对LASMMA系统手册进行了修约。