Assembling a system for measuring biological activity of micro-level particle in heterogenous system

建立了一套测量非均质系统中浮游生物、细菌细胞及其菌落等微观粒子生物活性的新系统。该系统被命名为LASMMA（激光半微探针质量分析），由YAG激光器、红外显微镜、石英池、气体纯化线和同位素比质谱仪（IRMS）组成。该系统专门用于浮游植物光合作用的^<13> c示踪实验。制备了激光通过红外显微镜物镜照射样品的光学系统。利用电视摄像机对激光治疗过程中的微斑进行了识别。已知其同位素比的纤维素滤料用激光处理30 ~ 60分钟。经过净化和压力测量后，用已知^<13>C含量的标准CO_2气体稀释CO_2。将气体引入IRMS测定了^<13>C含量。虽然LASMMA对纤维素的测定值低于标准法，但表明其精度足以用于示踪实验。田间试验采用LSMMA方法。测定了苏瓦湖微囊藻（Microcystis）的^<13>碳吸收活性。在显微镜下对其菌落进行鉴定，并用激光对其进行处理。其^<13>C含量由实验开始时的1.09原子%增加到孵育24h后的3.52原子%。^<13>C含量的变化与通常方法测量的颗粒有机碳（POC）几乎相同。验证了LASMMA方法可用于现场示踪试验。此外，LASMMA系统可能不局限于示踪剂实验，尽管测量的标准偏差比常规方法大，测量时间长。为了将来的应用，LASMMA系统手册被四舍五入。

New system for measuring the biological activity of micro-level particles, for example, planktonic and bacterial cells and their colony, in heterogeneous system was developed. This system, namaed LASMMA (Laser Semi-Microprobe Mass Analysis), is composed of YAG laser, infra-red microscope, quartz cell, gas purification line, and isotope ratio mass spectrometer (IRMS). This system was specialized for the ^<13>C-tracer experiment on phytoplanktonic photosynthesis.Optical system, by which laser beam was applied to the sample through object lens of IR microscope, was prepared. Using TV camera, micro-spot was identified during laser treatment. Cellulose filter, of which isotope ratio was known, was treated with laser for 30 to 60 minutes. After purification and manometric measurement, the CO_2 was diluted with standard CO_2 gas of which ^<13>C content was known. The gas was introduced into IRMS to determine the ^<13>C content. Although the value of cellulose by LASMMA was lower than that by standard method, it suggested that its precision was enough for tracer experiment.LSMMA method was applid in the field experiment. The ^<13>C uptake activity of Microcystis was etsimated in Lake Suwa. Colony of this alga was identified under microscope, and treated by laser. Its ^<13>C content increased from 1.09 atom% at the beginning of the experiment to 3.52 atom% after 24hr incubation. The ^<13>C content changed almost as same as that measured for particulate organic carbon (POC) by the usual way.It was confirmed that LASMMA method could be applicable for field tracer experiment. Moreover, it was suggested that LASMMA system might not be limited for tracer experiment, although the standard deviation measured was larger than usual way and the measurement took much time.For future application, LASMMA System manual was rounded off.