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Analysis for effct of the ratios of CMV RNA species on the viral multiplicalion in tobacco tissues

烟草组织中CMV RNA种类比例对病毒增殖的影响分析

基本信息

批准号：
06660058
负责人：
OHKI Satoshi T
金额：
$ 1.34万
依托单位：
Osaka Prefecture University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06660058/
关键词：
Cucumber mosaic virus RNA Viral multiplication キュウリモザイクウイルス

项目摘要

Mechanism for the suppression of cucumber mosaic virus (CMV) multiplication is analyzed, with special reference to the green island tissue of mosaic leaves and the symptomless leaves periodically appear in the CMV-infected tobacco plants. Tobacco cv. Xanthi nc. was infected with a satellite-free isolate of CMV-pepo and analyzed for the early stage of infection. The symptomless leaves and the green tissue were resistant to the CMV inoculation with virus particles or viral RNA.Those tissues contained double stranded RNA 3 and 4 of the CMV genome. They contained very low level of single stranded RNA of CMV.They contained infectivity and coat protein (CP) as low as 5% of those in the yellow tissue. Ratios for the volume of RNA 3 and 4 to RNA 1 and 2 in the virus particles recovered from the tissues on each leaf position varied along with the symptom types appeared on each leaves. Symptomless leaves appeared above the leaves with larger ratio of RNA 3 and 4 to RNA 1 and 2. Virus multiplication was severely suppressed in the green islands, however, it was recovered in the protoplasts prepared from the green tissue. From these results, resistance in the symptomless leaves or the green tissue is not considered to be caused by CP.Replication of CMV RNA might be specifically suppressed by some unknown mechanism. Larger volume of RNA 3 and 4 may cause to develop the symptomless leaves or the green islands.

本文分析了黄瓜花叶病毒（CMV）抑制烟草增殖的机理，特别是在受CMV感染的烟草植株中，出现了花叶的绿岛组织和周期性的无叶现象。烟草克桑西县用CMV-pepo的无卫星分离物感染，并分析感染的早期阶段。无叶组织和绿色组织对CMV病毒颗粒或病毒RNA的接种具有抗性，这些组织含有CMV基因组的双链RNA 3和4。它们含有极低水平的CMV单链RNA，其感染性和外壳蛋白（CP）含量仅为黄色组织的5%。从各叶位的组织回收的病毒粒子中的RNA 3和4与RNA 1和2的体积比沿着各叶上出现的症状类型而变化。无症状叶片出现在叶片上方，RNA 3和4与RNA 1和2的比例较大。病毒增殖在绿色岛中受到严重抑制，然而，在从绿色组织制备的原生质体中恢复。从这些结果可以看出，无叶或绿色组织中的抗性不是由CP引起的。CMV RNA的复制可能受到某种未知机制的特异性抑制。RNA 3和RNA 4的量越大，越容易形成无叶或绿色岛。