腎移植兼膵ランゲルハンス島移植での、両移植間の最適の移植時期を見い出す研究

肾移植与胰岛移植最佳移植时机的研究

• 批准号: 06770909
• 负责人: 古屋 智規
• 金额: $ 0.58万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06770909/
• 关键词: 膵ランゲルハンス島移植; 腎移植; 免疫学的エンハンスメント

1.実験デザインの検討:本実験では腎の長期生着が必要であるが,当初の計画である,純系ラットBNからLEWへの組み合わせでは,腎の生着期間が十分に得られなかった。そこで,免疫抑制の併用あるいは,他の組み合わせへの変更を検討した結果,主要組織適合抗原が同じで非主要組織適合抗原のみ異なるF344→LEWが適当と判断した。2.F344→LEWでのラ島/腎移植:上記組み合せにおいてラ島移植または腎移植を行い,生着日数を検討した。(1)腎臓の生着日数(生存日数)は中央値20日で,20日における生着率は60%であった。(2)ラ島移植:移植1日後の血糖は100mg/dl以下となった後,同レベルを維持,8日以降250mg/dl以上となった(生着日数中央値:8日)。(3)腎ラ島同時移植:腎の同時移植にて,ラ島の生着日数延長の程度を確認中である。3.免疫学的および組織学的検索:HEおよび免疫学的組織学的検索にてラ島の易拒絶性を確認した。4.今後の予定,及び課題:現在腎同時移植によってラ島の生着率改善を証明しつつあり,免疫学的エンハンスメントが得られるものと考えられた。今後,腎移植の時期を変えて,最適の移植時期を検討,エンハンスメントと感作との関連を明らかにする予定である。その後,さらにlymphocytotoxic antibody titer,MLR等の免疫学的検索を行い,その機序解明を検討する計画である。

1. Discussion on the development of kidney disease: This is the first time that kidney disease has been necessary for a long time. In the original plan, pure BN disease has been established. The results of the study showed that the main tissues were suitable for the same antigen and the non-main tissues were suitable for the different antigens. F344→LEW was suitable for the judgment. 2. F344 →LEW: The above group is divided into two groups: the first group is divided into two groups: the second group is divided into two groups: the first group is divided into three groups: the first group is divided into three groups: the second group is divided into three groups: the first group is divided into three groups: the second group is divided into three groups: the third group is divided into four groups: the fourth group is divided into three groups: the fourth group is divided into four groups: the fourth group is divided into (1)The number of days of kidney birth (days of survival) is 20 days, and the rate of kidney birth is 60% on the 20th. (2)After transplantation, blood glucose decreased to 100mg/dl or less 1 day after transplantation, and then decreased to 250mg/dl or more 8 days after transplantation (central value of birth days:8 days). (3)Simultaneous kidney transplantation: Simultaneous kidney transplantation, confirmation of the extent of extension of the number of days of life of the kidney island. 3. Immunological and Histological Studies:HE and Immunological Histological Studies confirm the susceptibility of the disease. 4. Future projections and issues: proof of improvement in survival rate of simultaneous kidney transplantation, immunology, etc. In the future, the timing of kidney transplantation will be changed, and the optimal transplantation timing will be discussed. In the future, we will continue to investigate the immunology of lymphocyte cytotoxic antibody titers,MLR, etc., and plan to investigate the mechanism of the disease.