未熟児網膜症における網膜硝子体新生血管の発生機序並びに治療に関する実験的研究

早产儿视网膜病变玻璃体视网膜新生血管形成机制及治疗的实验研究

• 批准号: 06771508
• 负责人: 重藤 真理子
• 金额: $ 0.51万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06771508/
• 关键词: 高濃度酸素暴露; 未熟児網膜症; 網膜新生血管

高濃度酸素80%を7日間,14日間,Brom Nourayの新生児ラットに暴露させた後それぞれ大気中に旁し3w飼育後に、眼球の検査を行った。酸素投与期間14日間のものに網膜新生血管が認められた。酸素の投与濃度を40%〜80%と変動させたものでは新生血管は認められなかった。実体顕微鏡、光学顕微鏡、電子顕微鏡で観察し病理学的に新生血管を確認し、新生児の高濃度酸素投与による網膜新生血管の発生、つまり未熟児網膜症のモデルとして活用できることがわかった。尚、新生血管の発症は様々な眼疾患に伴うもので様々な血管増殖因子、抑制因子の反応をみて良いモデルとなる。今年度はモデル作製と病理学的観察、増殖の場である硝子体コラーゲンの免疫染色等を行った。又、DMや頚動脈狭窄を人為的に加え、増殖性変化を増強させる実験を行った。(H6年5月九州眼科学会H5年4月小児眼科学会で発表)

In the period of 80% to 7 days and 14 days after exposure to high concentration of acidity, Brom Nouray newborn babies were exposed to postnatal pregnancy, and their eyeballs were examined after 3 weeks of pregnancy. During the period of 14 days, the neovascularization of the reticular membrane was induced. The concentration of acid is 40%, 80%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, 40%, 80%, Microscopes, optical microscopes, and electronic microscopes were used to detect the confirmation of neovascularization in pathology, the injection of high levels of acid in neovascularization and the formation of neovascularization in the neovascularization of the network, and the diagnosis of immature network disease. Still, neovascularization syndrome, ophthalmopathy, concomitant angiogenesis, angiogenesis factor, inhibitory factor, anti-angiopathy, anti-angiogenesis, neovascularization, neovascularization, ophthalmopathy, neovascularization, angiogenesis, angiogenesis, neovascularization, ophthalmopathy, eye disease, angiogenesis, neovascularization, ophthalmopathy, ophthalmopathy. This year, we have made a pathological survey of the disease, such as the observation of pathology, the production of nitrate bodies, the immune staining and so on. In addition, the DM campaign to reduce the use of human-made drugs, reproductive diseases, and strengthen the operation of the public health care system. (may H6, Kyushu Ophthalmology Society H5, April 5, Kyushu Ophthalmology Society)