ANALYSES OF GENOMIC IMPRINTING IN CHROMOSOME TRANSLOCATIONS OF HEMATOLOGIC NEOPLASIA

血液肿瘤染色体易位基因组印记分析

• 批准号: 06671100
• 负责人: TOMONAGA Masao
• 金额: $ 1.34万
• 依托单位: NAGASAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06671100/
• 关键词: genomic imprinting; t (8 ; 21); t (15 ; 17); t (9 ; 22); C-banding method; Ag-I-staining method; 白血病; 染色体転座; -

It has been controversial in Ph-positive chronic myeloid leukemia (CML) whether the rearranged chromosomes 9 and 22 have 'parent of origin'bias. In this study, we investigated parental origin not only of rearranged chromosomes 9 and 22 in Ph-positive CML by C-banding and Ag-I-staining methods, respectively, but also of rearranged chromosome 21 in t (8 ; 21) -positive acute myeloid leukemia (AML) and of rearranged chromosome 15 in t (15 ; 17) -positive AML by Ag-I-staining method. As a result, it was disclosed that among five Ph-positive CML patients one had paternal rearranged chromosomes 9 and maternal rearranged chromosome 22, one maternal rearranged chromosomes 9 and paternal rearranged chromosome 22, and one undetermined rearranged chromosomes 9 and maternal rearranged chromosome 22. Among seven t (8 ; 21) -postitive AML patients, one had paternal rearranged chromosome 21 and three maternal one. Among six t (15 ; 17) -positive AML patients, two had paternal rearranged chromosome 15 and one maternal one. In 1992, Haas et al.indicated that in Ph-positive CML rearranged chromosomes 9 and 22 are exclusively paternal and maternal in origin, respectively, by C-banding and Ag-I-staining methods. However, several reports which investigated the parental origin of the rearranged chromosomes by molecular methods did not support the Haas's hypothesis. Our results indicate that there is no parental bias, i.e., genomic imprinting, in the origin not only of ABL and M-BCR which are rearranged in Ph-positive CML,but also of AML-1 and PML in t (8 ; 21) -and t (15 ; 17) -associated AMLs, respectively.

在Ph阳性的慢性粒细胞白血病(CML)中，9号和22号染色体的重排是否存在“起源亲本”的偏向一直存在争议。在本研究中，我们不仅用C显带和银染方法研究了Ph阳性CML中9号和22号染色体重排的亲本来源，而且还用银染方法研究了t(8；21)阳性急性髓细胞白血病(AML)中21号染色体重排和t(15；17)阳性AML中15号染色体重排的亲本来源。结果发现，在5例Ph阳性的CML患者中，1例存在父亲9号染色体重排和母方22号染色体重排，1例母方9号染色体重排和22号染色体重排，1例9号染色体和22号染色体未确定重排。在7例t(8；21)阳性的AML患者中，1例为父系21号染色体重排，3例为母系重排。在6例t(15；17)阳性AML患者中，2例父系15号染色体重排，1例母系重排。1992年，Haas等人用C显带和Ag-I染色的方法指出，在Ph阳性的CML中，重排的9号和22号染色体分别为父系和母系。然而，一些用分子方法研究重排染色体亲本来源的报道并不支持Haas的假说。我们的结果表明，不仅在Ph阳性的CML中重排的ABL和M-BCR的起源中没有亲本偏见，即基因组印迹，而且在t(8；21)和t(15；17)相关的AML中AML-1和PML的起源也没有亲本偏见。

项目成果

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Y.Shimamoto：“用全反式维甲酸治疗急性早幼粒细胞白血病后高组胺血症症状的预防”《血液学报》。

中村 秀男: "Morphological subtyping of acute leukemia with maturation (AML-M2) : homogeneous pink-colored cytoplasm of mature neutrophils is most charateristic of AML-M2 with t(8;21)." LEUKEMIA. (発表予定).

Hideo Nakamura：“成熟型急性白血病 (AML-M2) 的形态学亚型：成熟中性粒细胞的均匀粉红色细胞质是 t(8;21) AML-M2 的最大特征。”

Dale L.Preston: "Cancer Incidence in Atomic Bomb Survivors. Part III:Leukemia,Lymphoma and Multiple Myeloma, 1950-1987" RADIATION RESEARCH. 137. 68-97 (1994)

Dale L.Preston：“原子弹幸存者的癌症发病率。第三部分：白血病、淋巴瘤和多发性骨髓瘤，1950-1987 年”辐射研究。

Hideo Nakamura, Kazutaka Kuriyama, Naoki Sadamori, Mariko Mine, Takahiro Itoyama, Ippei Sasagawa, Kazuhiro Matsumoto, Yoshiro Tsuji, Norio Asou, Shin-IchiKageyama, Hisashi Sakamaki, Nobuhiko Emi, Ryuzo Ohno, and Masao Tomonaga: "Morphological Subtyping of

Hideo Nakamura、Kazutaka Kuriyama、Naoki Sadamori、Mariko Mine、Takahiro Itoyama、Ippei Sasakawa、Kazuhiro Matsumoto、Yoshiro Tsuji、Norio Asou、Shin-IchiKageyama、Hisashi Sakamaki、Nobuhiko Emi、Ryuzo Ohno 和 Masao Tomonaga：“形态学子类型化

中村秀男: "Morphological subtyping of acute myeloid leukemia with maturation(AML-M2) : homogeneous pinkcolored cytoplasm of mature neutrophils is most charateristic of AML-M2 with t(8;21)." LEUKEMIA. (in press).

Hideo Nakamura：“成熟型急性髓性白血病 (AML-M2) 的形态学亚型：成熟中性粒细胞的均质粉红色细胞质是 t(8;21) AML-M2 的最具特征性。”