A Research for, cellular Dysfunction in Acta Stroke

Acta 中风细胞功能障碍的研究

• 批准号: 06671385
• 负责人: KANAMARU Kenji
• 金额: $ 1.22万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06671385/
• 关键词: Nitroglycerin; Nitric Oxide (NO); Subarachnoid Homorrhage; Vasospasm; cGMP; Hyperpolarization; Protein S; AntithrombinIII; 脂質過酸化反応; 脳血管攣縮; 細胞膜過分極; パッチクランプ; 正中縫線核細胞; 網膜神経節細胞; whole-cell curent clamp; whole-cell voltage clamp; potassium curr

Nitroglycerin, as well as nitric oxied, causes hyperpolarization and cGMP elevation in vascular smooth muscle cells. It is unknown whether nitroglycerin ameliorates vasospasm by an increase in cGMP levels after sudarachnoid hemorrhage (SAH). The purpose of the first study was to measure the levels of cGMP and cAMP in the cerebral arteries and parietal cerebral cortices in a primate model and to determine the effect of nitroglycerin on vasospasm after SAH.Chronic vasospasm was induced by clot placement around the right middle cerebral artery (MCA). Seven days after the surgery, angiography was repeated and either nitroglycerin or saline was administered intrevenously. A significant vasospasm occurredin the cerebral arteries on both sides, more prominently on the right side. Concomittantly, rCBF on the right side was significantly decreased. In the right MCA,cGMP levels were significantly lower than in the normal MCA.After the administration of nitroglycerin for 3 hours, the cerebral vessels were significantly dilated on both sides, and rCBF was singnificantly increased on the right side but not on the left side. Although depressed cGMP levels in the right MCA were not recovered by nitroglycerin, a significant increase in cGMP levels was observed in the basilar artery. In both parietal cortices, cGMP levels were significantly decreased after SAH and unchanged after nitroglycerin treatment. There were no significant changer in cAMP levels in SAH and after nitroglycerin treatment. In conclusion, the vasodilator effect of nitroglycerin in spastic MCA may not be mediated by an increase in cGMP levels, suggesting an involvement of hyperpolarization of the smooth muscle cells.

硝酸甘油和一氧化氮引起血管平滑肌细胞超极化和cGMP升高。目前尚不清楚硝酸甘油是否通过增加蛛网膜下腔出血(SAH)后cGMP水平来缓解血管痉挛。第一项研究的目的是在灵长类动物模型中检测大脑动脉和顶叶皮质中cGMP和cAMP的水平，并确定硝酸甘油对SAH后血管痉挛的影响。术后7天重复血管造影术，静脉注射硝酸甘油或生理盐水。双侧脑动脉均出现明显的血管痉挛，右侧更为明显。同时，右侧rCBF显著降低。给予硝酸甘油3h后，双侧脑血管明显扩张，右侧rCBF显著增加，左侧无明显变化。虽然硝酸甘油不能使右侧大脑中动脉cGMP水平恢复，但基底动脉cGMP水平显著升高。SAH后双侧顶叶皮质cGMP水平显著降低，硝酸甘油治疗后无明显变化。SAH患者及硝酸甘油治疗后cAMP水平无明显变化。结论：硝酸甘油对痉挛MCA的血管扩张作用可能不是通过增加cGMP水平实现的，提示可能参与了血管细胞的超极化。

项目成果

金丸 憲司: "Effects of vasospasm on heme oxygenases in a rat model of subarachnoid hemorrhage" Stroke.

Kenji Kanamaru：“血管痉挛对蛛网膜下腔出血大鼠模型中血红素氧合酶的影响”中风。

Kenji Kanamaru: "Effects of Nitroglcecerin on vasospasm and cyclic maclotidasb in a primate model of subarachnoid nemorrhage" Stroke. 27. 1882-1888 (1996)

Kenji Kanamaru：“硝化甘油对蛛网膜下腔出血灵长类动物模型中血管痉挛和环状 maclotidasb 的影响”中风。

金丸憲司: "Plasma levels of ProteinC,ProteinS,and antithrombiIII in patients with subarachnoid hemorrhage." Acta Neurochirurgica. (in press). (1997)

Kenji Kanemaru：“蛛网膜下腔出血患者的蛋白质 C、蛋白质 S 和抗血栓 III 的血浆水平”（Acta Neurochirurgica）（1997 年出版）。

金丸憲司: "Effects of nitroglycerine on Vasospasm and cyclic nucleotides in a primate model of subarachnoid hemorrhage" Stroke. 27. 1882-1888 (1996)

Kenji Kanamaru：“硝化甘油对蛛网膜下腔出血灵长类动物模型中血管痉挛和环核苷酸的影响” 27. 1882-1888 (1996)

金丸 憲司: "Plasma levels of Protein C, Protein S, and antithrombinIII in patibuts with. subarachnoid hemorrhage" Acta Nenrochir (Wien). 139. 134-140 (1997)

Kenji Kanamaru：“蛛网膜下腔出血患者中蛋白质 C、蛋白质 S 和抗凝血酶 III 的血浆水平”Acta Nenrochir (Wien) 139. 134-140 (1997)。