Genes involved in decidualization of endometrium

人类子宫内膜经历了一个戏剧性的变化，即“去个体化”，包括增殖和分化，并伴有成功胚胎着床所需的一些阶段特异性基因的诱导。子宫内膜的脱体细胞化包括间质内的成纤维细胞分化为上皮样细胞和活跃的内皮细胞。已经确定了几个子宫内膜去个体化的生化指标，包括催乳素、妊娠相关血浆蛋白A、胎盘蛋白12、胎盘蛋白14和表皮生长因子。由于编码这些指示蛋白的基因是在去个体化后表达的，因此它们必须表达分化过程的后一部分。本研究的目的是寻找可能调控人子宫内膜间质细胞脱质化发生的转录因子，如POU同源基因。以POU结构域基因家族保守序列为基础，利用引物对逆转录-聚合酶链反应，从人早孕蜕膜总RNA中扩增出参与人蜕膜化的POU结构域基因cDNA。扩增产物插入pCR II载体，测序。部分克隆序列与Oct-3序列一致。我们检测了Oct-3mRNA在月经周期和妊娠早期的变化。Oct-3 mRNA在黄体期和妊娠早期升高。Oct-3 mRNA的表达与这些细胞体内脱体细胞化的开始一致。人子宫内膜基质细胞在体外黄体酮作用下分化为蜕膜细胞。添加黄体酮24 h后Oct-3 mRNA升高。在体外实验中，添加反义寡核苷酸对黄体酮诱导的去个别化有抑制作用。

Human endometrium undergoes a dramatic changes, "decidualization", that includes proliferation and differentiation accompanied by induction of a number of phase specific genes required for successful embryonal implantation. The decidualization of the endometrium involves differentiation of fibroblastic elements within the stroma into epithelioid and active endocrime decidual cells. Several biochemical indicators of endometrial decidualization have been identified, including prolactin, pregnancy associated plasma protein A,placental protein 12, placental protein 14 and epidermal growth factor. Since the genes encoding these indicator proteins are expressed after decidualization, they must express the latter part of this differentiation process. The aim of the present study was to search for transeriptional factor, such as POU homeotic genes, which may regulate the onset of decidualazation of human endometrial stromal cells.cDNA of POU domain genes involved in human decidualazation were amplified from human early pregnant decidua total RNA using by reverse transcription-pilymerase chain reaction with primer pair, which were constructed based on conservative sequences of POU domain gene family.Amplified products were coned into pCR II vector and sequenced. The sequence of some clones corresponded with that of Oct-3. We exmined the changes of Oct-3mRNA during menses cycle and early pregnacy. Oct-3 mRNA was increased in luteal phase and early pregnancy. Oct-3 mRNA expression coincides with the onset of decidualization of these cells in vivo. Human endometrial stromal cells were differentiated into decidual cells in vitro in the presence of progesterone. Oct-3 mRNA was increased 24 hours after progesterone addition. The addition of antisence oligonucleotide was inhibited progesterone induced decidualization in vitro.