上腸間膜動脈閉塞・再灌流時の腸管運動に関する検討

肠系膜上动脉闭塞/再灌注过程中肠道蠕动的研究

• 批准号: 06857082
• 负责人: 川村 弘之
• 金额: $ 0.58万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06857082/
• 关键词: 小腸運動; 虚血再灌流; strain gage force trausclucer; S-100蛋白

1.虚血再灌流時の腸管運動(非虚血時の腸管運動)5-10分間隔で単発性,spike様の収縮を認めた.(虚血時の運動)虚血5分以内に次第に増強漸減する高周波の痙攣様の強収縮波形が認められた.また,その強収縮は徐々に減弱し30分以内に消失した.(再灌流時の運動)30,60分虚血群の全例および90分虚血の一部の症例において再灌流後10-20分後よりspike様の収縮波形が散発性に出現した.出現間隔は不規則であったがその後も持続した.その他の90分虚血および120分虚血群においては収縮波形の出現は認められなかった.2.エネルギー代謝・腸管運動の比較検討ATPの変動においては虚血後30分間に急速に低下し,虚血前に比し約30%にまで低下し,その後緩慢に低下した.この変動は腸管運動の振幅の増減とよく相関するものであり興味ある結果であった.再灌流後のATPの回復程度は虚血時間と負の相関を示したが,再灌流後の収縮波の出現の有無との関係に解明にはさらなる検討が必要と思われる.腸管運動における各種薬剤の反応カルシウム拮抗薬に関しては虚血時の強収縮は有意に抑制された.同時にATPの虚血時における低下の抑制,再灌流時における良好な回復を認めた.ネオスチグミンは非虚血時において腸管の収縮を惹起した.また,再灌流時においては30,60分虚血の全例および90分虚血の一部の症例で収縮を惹起し腸管のviabilityとの関係を示唆する所見と考えた.4.組織学的検討HE染色では虚血時間が延長するに従って浮腫,絨毛の脱落,出血が認められた.S-100蛋白染色では神経線維とグリア細胞の染色性は時間の経過とともに低下し,120分ではほぼ消失していた.虚血60分以上では神経線維の膨化とグリア細胞核の空胞化および核の変形など細胞変性,壊死の変化がみられ,虚血時間とともに顕著となり,120分後には神経節細胞も変性・壊死を呈していた.

1. Intestinal movement during reperfusion with deficient blood (intestinal movement during non-deficient blood)5-10 minute intervals, spikes and contractions. (Movement in case of deficiency of blood) Within 5 minutes of deficiency of blood, the intensity gradually increases and decreases, and the high frequency wave spasms and intense contraction waveforms are recognized. The intensity of the contraction decreases and disappears within 30 minutes. (Movement during reperfusion) 30, 60 minutes All cases of deficient blood group were more than 90 minutes, some cases of deficient blood group were more than 10-20 minutes after reperfusion. Irregular intervals appear. 2. Metabolize and compare the changes of ATP in intestinal tract movement. After 90 minutes of blood deficiency, ATP decreased rapidly. Before blood deficiency, ATP decreased by about 30%. After that, ATP decreased slowly. The amplitude of the motion of the intestine decreases. The degree of ATP recovery after reperfusion is negatively correlated with the time of blood loss, and the relationship between the occurrence of contraction wave after reperfusion and the occurrence of contraction wave is discussed. Intestinal motility is affected by the adverse reactions of various agents, which are related to the inhibition of blood circulation. At the same time, ATP is suppressed when blood is deficient, and a good recovery is achieved during reperfusion. When the blood is not empty, the intestine shrinks. 4. Histological examination: HE staining: prolonged duration of ischemia, edema, shedding of villi, bleeding, S-100 protein staining: decreased staining time of neurons, decreased staining time of cells, decreased staining time of cells. 120 minutes, When the blood deficiency score is over 60 minutes, the nerve cell dimension expands, the nucleus becomes empty, the nucleus becomes abnormal, the nucleus becomes abnormal, the death becomes abnormal, the blood deficiency time becomes abnormal, 120 minutes later, the nerve cell becomes abnormal, the death becomes abnormal.