Structure and function of steptomyces RNA polymerase

链霉菌RNA聚合酶的结构和功能

• 批准号: 07044316
• 负责人: FUJITA Nobuyuki
• 金额: --
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for International Scientific Research.
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044316/
• 关键词: Streptomyces; RNA polymerase; Transcription regulation; Cell differentiation; Promoter

Steptomyces has a unique life cycle with a complex differentiation in morphology and physiology.On solid media, the bacteria grow as substrate mycelia which extend to form aerial hyphae and ulitimately differentiate into exospores.At certain plases during the sporulation, they produce varieties of antibiotics.From the knowledge of gene expression control in E.coli, the modulation of promoter selectivity of RNA polymerase must be involved in this switching control of gene transcription in Streptomyces.In order to test this possibility, RNA polymerase was purified for the first time from S.greseus and analyzed for its structure and function.The enzyme from vegetative-phase mycelia was found to contain a principal sigma factor encoded by the gene hrdB and to transcribe some essential genes for cell growth.Thus, we concluded that Esigma-B (or E-HrdB) is the major RNA polymerase holoenzyme in the exponentially growing cells.In parallel with the analysis of RNA polymerase, a collection of S.griceus promoters is being made for future analysis of minor sigma factors.

链霉菌具有独特的生活史，在形态和生理上具有复杂的分化。在固体培养基上，该细菌作为底物菌丝体生长，形成气生菌丝，并最终分化为外壁。在产孢期的某些阶段，它们产生各种抗生素。从大肠杆菌基因表达调控的知识来看，RNA聚合酶启动子的选择性调控必须参与链霉菌基因转录的开关控制。为了验证这种可能性，首次从格氏链霉菌中分离纯化了RNA聚合酶，并对其结构和功能进行了分析。从营养生长期的菌丝体中发现，该酶含有一个由hrdb基因编码的主要西格玛因子，并转录了一些细胞生长所必需的基因。因此，我们得出结论：Esigma-B(或E-hrdb)是指数生长期细胞中主要的RNA聚合酶。

项目成果

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Ishihama, A.：“细菌稳定期生存的遗传策略。”

Lezhava,A.,Mizukami,T.,Kajitani,T.,Kameoka,M.,Redenbach,M.,Shinkawa,H.,Niimi,O.and Kinashi,H.: "Physical map of the linear chromosome of Streptomyces griseus" J.Bacteriol. 177. 6492-6498 (1995)

Lezhava，A.，Mizukami，T.，Kajitani，T.，Kameoka，M.，Redenbach，M.，Shinkawa，H.，Niimi，O.和Kinashi，H.：“灰色链霉菌线性染色体的物理图

Shinkawa, H., Fijita, N., Shiina, T., Tanaka, K., Takahashi, H., Ishihama, A.and Nimii, O. :"Purification and characterization of RNA polymerase holoenzyme (Esigma^B) from vegetative-phase mycelia of Streptomyce griseus." J.Biochem.118. 488-493 (1995)

Shinkawa, H.、Fijita, N.、Shiina, T.、Tanaka, K.、Takahashi, H.、Ishihama, A. 和 Nimii, O.：“植物中 RNA 聚合酶全酶 (Esigma^B) 的纯化和表征

Shinkawa,H.,Fujita,N.,Shiina,T .,Tanaka,K.,Takahashi,H.,Ishihama,A.and Nimii,O.:"Purification and characterization of RNA polymerase holoenzyme(Eσ^B)from vegetative-phase mycelia of Streptomyce griseus." J.Biochem.118. 488-493 (1995)

Shinkawa, H.、Fujita, N.、Shiina, T.、Tanaka, K.、Takahashi, H.、Ishihama, A. 和 Nimii, O.：“植物中 RNA 聚合酶全酶 (Eσ^B) 的纯化和表征灰色链霉菌的-期菌丝体。”J.Biochem.118. 488-493 (1995)

Shinkawa, H., Hatada, Y., Okada, H., Kinashi, H.and Niimi, O.: "Nucleotide sequence of a principal sigma factor gene (hrdB) of Streptomyces griseus" J.Biochem.118. 494-499 (1995)

Shinkawa, H.、Hatada, Y.、Okada, H.、Kinashi, H. 和 Niimi, O.：“灰色链霉菌主要 σ 因子基因 (hrdB) 的核苷酸序列”J.Biochem.118。