Basic and clinical aspect of immune-mediated inner ear disorders

免疫介导的内耳疾病的基础和临床方面

• 批准号: 07307031
• 负责人: MOGI Goro
• 金额: $ 3.2万
• 依托单位: Oita Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07307031/
• 关键词: autoimmune diseases; PCR (polymerase chain reaction); HLA (human leukocyte antigen); adhesion molecule; Na, K-ATPase; spiral ligament; endolymphatic sac; anaphylaxis; ステロイド依存性難聴; HLA (human leukocyte antigen); PCR法; ICAM-1; 血液-内耳関門; 内リンパ曩; 増

A.Clinical Studies(1) The clinical features of steroid-responsive sensorineural hearing loss and acute sensorineural hearing loss with autoimmune diseases were investigated.(2) Using a RT-PCR technique, the measles virus was detected in perilymph taken during cochlear implant surgery in patients with profound hearing loss.(3) The frequency of HLA-Aw33 in otosclerosis patients was higher than that in the control subjects.B.Basic Studies(1) Secondary antigen challenge into the endolymphatic sac resulted in the cellular migration and ICAM-1 expression in the whole inner ear venules, suggesting that chemical mediators produced within the endolymphatic sac may spread into all compartments of the inner ear and lead to immunological defense and injury of the inner ear.(2) Inner ear responses to foreign cells induced activation and invasion of natural killer cells which occur relatively late compared with those in other organs.(3) Decreased immunoreactivity for Na, K-ATPase and connexin 26 was observed in the spiral ligament after immune injury.(4) Study of cell proliferation revealed that the epithelium of the endolymphatic sac can regenerate after immune injury.(5) Repeated antigen challenges into the inner ear caused an anaphylactic reaction that may disturb hearing and equilibrium.(6) MRL/prmouse and NZB mouse were studied electrophysiologically and histopathologically to clarify the mechanism by which autoimmune inner ear diseases occur.

A.临床研究（1）探讨类固醇反应性听力损失和自身免疫性疾病引起的急性听力损失的临床特征。（2）采用RT-PCR技术，在重度听力损失患者人工耳蜗植入手术时抽取的外淋巴中检测到麻疹病毒。（3）耳硬化症患者中HLA-Aw33的频率高于对照组 B.基础研究(1)内淋巴囊的二次抗原攻击导致整个内耳小静脉的细胞迁移和ICAM-1表达，表明内淋巴囊内产生的化学介质可能扩散到内耳的所有区室，导致内耳的免疫防御和损伤。(2)内耳对外来细胞的反应诱导自然杀伤细胞的激活和侵袭，发生得相对较晚 与其他器官相比。(3)免疫损伤后，螺旋韧带中观察到Na、K-ATP酶和连接蛋白26的免疫反应性降低。(4)细胞增殖研究表明，内淋巴囊上皮在免疫损伤后可以再生。(5)反复向内耳激发抗原会引起过敏反应，可能会干扰听力和听力损失。 (6)对MRL/prmouse和NZB小鼠进行电生理学和组织病理学研究，阐明自身免疫性内耳疾病发生的机制。

项目成果

神崎 仁: "免疫異常に関連する感音難聴-厚生省調査研究班診断基準(案)の見通しと今後の問題" Otol Jpn. 6. 151-156 (1996)

Hitoshi Kanzaki：“与免疫异常相关的感觉神经性听力损失 - 厚生省研究小组诊断标准（草案）的前景和未来问题” Otol Jpn. 6. 151-156 (1996)

H.Yamane Y.Nakai et al.: "In vivo regeneration of vestibular hair cells of guinea pig" Acta Otolaryngol (Stockh). S520. 174-177 (1995)

H.Yamane Y.Nakai 等人：“豚鼠前庭毛细胞的体内再生”Acta Otolaryngol (Stockh)。

神崎 仁: "ステロイド依存性難聴-突発性難聴との鑑別-" Otol Jpn. 4(2). 146-153 (1994)

Hitoshi Kanzaki：“类固醇依赖性听力损失 - 与突发性听力损失的区别 -”Otol Jpn 4(2) (1994)。

神崎 仁: "免疫異常による感音難聴をどのように診断するか 耳鼻咽喉科頭頚部外科クリニカルトレンド" 中山書店,野村恭也,本庄 巌,平出文久 編, 285 (1996)

Hitoshi Kanzaki：“如何诊断因免疫异常导致的感音神经性听力损失：耳鼻咽喉头颈手术的临床趋势” Nakayama Shoten、Kyoya Nomura、Iwao Honjo、Fumihisa Hiraide 编辑，285 (1996)

蓑田涼生、宇野研吾 他: "能動感作内耳I型アレルギーモデルにおける抗原投与後の聴力変動と眼振の出現について" Equilibrium research. 55(印刷中). (1996)

Ryoo Minota、Kengo Uno 等人：“关于在主动致敏耳蜗 I 型过敏模型中施用抗原后出现的听力波动和眼球震颤”平衡研究 55（出版中）。