STUDIES ON MOLECULAR MECHANISMS AND PHYSIOLOGICAL FUNCTIONS OF ANTI-CELL ADHESION

一些已建立的细胞系对硫酸软骨素蛋白多糖的抗黏附活性不敏感，另一些细胞系缺乏具有抗黏附活性的硫酸软骨素链的候选受体Annexin VI。这些可能有助于分析Annexin VI在抗黏附(Kimata)中的可能作用。HSP-47反义RNA的表达不仅导致胶原合成减少，而且胶原不溶解，提示HSP-47作为Shaperon(Nagata)的作用。北川分析了突变的层粘连蛋白基因对细胞行为的影响。Tenascin产生活性高的乳腺癌细胞与低活性的乳腺癌细胞相比，细胞形态圆整，细胞迁移活性高，侵袭力强，提示Tenascin的抗黏附活性对转移(Sakakura)有一定的影响。此外，与抗粘连不同的是，RGD型独立…更多的凹痕整合素可能参与了Tenascin(Obara)的一种隐蔽的细胞黏附活性。神经多糖C是一种新的膜结合型蛋白多糖，它影响大鼠脑细胞的黏附，并可能诱导神经突起生长(Oohira)。低转移活性的肺癌细胞通常表现为Syndecan的高表达，并倾向于形成有组织的应力纤维，而高转移活性的肺癌细胞则倾向于诱导褶皱膜的形成。这种差异可能解释了细胞粘附性和癌细胞转移能力之间的可能关系(Okayama)。Sekiguchi发现，纤维连接蛋白的选择性剪接可以调节细胞黏附能力。一种具有酪氨酸磷酸酶活性的脑蛋白多糖被发现具有与神经中枢神经细胞的抗粘连活性。这种蛋白多糖可能作为多营养素(Noda)的受体发挥作用。Sofue提出层粘连蛋白可能是IGF-I依赖的分化诱导因子，并进一步发现酪氨酸磷酸化在信号转导中的作用。稻垣已成功地将单抗提高到磷酸化的细胞骨架蛋白，并揭示了中间丝的磷酸化与增殖细胞的细胞黏附变化的可能关系。Tsukita观察到，当钙粘附素结合蛋白b-catenin被迫表达时，整合素被激活。第一次和第二次小组会议在名古屋举行，讨论结果，相互提出有用的建议和评论，并交流最新信息。较少

Some of established cell lines are insensitive to anti-adhesive activity of chondroitin sulfate proteoglycans, and other lines are defective of annexin VI which is a candidate of the receptor for chondroitin sulfate chains with anti-adhesive activity. Those may be useful to analyze a possible role of annexin VI in anti-adhesion (Kimata). Transfection with antisense RNA for HSP-47 expression caused not only the decrease in collagen synthesis but also insolubilization of the collagen, which suggests a role of HSP-47 as shaperon (Nagata). Kitagawa analyzed the effect of the transfection with mutant laminin genes on the cell behaviors. Mammary carcinoma cells with high activity of tenascin production, compared to those with the low activity, showed the round cell morphology, the high activity of cell migration and the high invasiveness, which sugggests the effect of anti-adhesive activity of tenascin on the metastasis (Sakakura). In addition, differently from the anti-adhesion, RGD-indepen … More dent integrin may be involved in a cryptic cell-adhesive acitivity of tenascin (Obara). A novel membrane-bound proteoglycan, neuroglycan C influences cell adhesion in rat brain and may induce the neurite outgrowth (Oohira). lung carcinoma cells with the low metastatic activity usually show the high expression of syndecan and tend to show the organized stress fiber formation, but those with the high activity tend to induce the formation of ruffling membranes. The difference may explain a possible realationship between the cell adhesiveness and the metastatic ability of the carcinoma (Okayama). Sekiguchi has found that altemative splicing of fibronectin modulates the capacity for cell-adhesion. A brain proteoglycan with tyrosine phosphatase activity has been found to exhibit anti-adhesion activity to neuronal cells in the nervous center. This proteoglycan may function as a receptor for pleiotrophin (Noda). Sofue has suggested that laminin may be a IGF-I-dependent differentiation-inducing factor and further found the inbolvement of tyrosine phosphorylation in the signal transduction. Inagaki has succeeded in raising monoclonal antivodies to phosphorylated cytoskeletal proteins and revealed the possible relationship of the phosphorylation of intermediate filaments to the changes in cell-adhesion of proliferating cells. Tsukita has observed the activation of integrin when a cadherin-binding protein, b-catenin is forced to be expressed. The first and second group meetings were held in Nagoya to discuss results, give useful suggestions and comments each other and exchange current informations. Less