Chiral Recognition of Host Molecules Determined by Mass Spectrometry

在过去的一年中，我们成功地发展了一种新的方法，我们称之为“FABMS/EL（Enantiomer-Labeled）客体方法”，并阐明了这种新方法的基本方面。通过IRIS值：I [（H+G_R）^+] /I [（H+G_）^+]来评价手性识别能力<S-dn>。今年，我们（i）将该方法应用于各种主体分子，（ii）将该方法扩展到ESI（Electrospray Ionization）质谱，即“ESIMS/EL客体方法”。(1)“FABMS/EL客体法”的结果表明，在IRIS=5.0-0.2范围内，各种手性冠醚、糖类和抗生素对各种氨基酸及其酯表现出不同程度的手性识别。(2)“ESIMS/EL客体法”的结果表明，与FABMS相比，ESIMS的IRIS值有一定的降低，因此在定量评价手性识别能力时应谨慎。

In the last year, we coult successfully develop a new methodology for chiral recognition in the highly structured host-guest complexation systems, which we called as "FABMS/EL (Enantiomer-Labeled) guest method", and clarify the fundamental aspects of this newly developed method. Chiral recognition ability was evaluated by the IRIS values : I [(H+G_R) ^+] /I [(H+G_<S-dn>) ^+].In this year, (i) we applied this method to various host molecules, and (ii) we extended this method to ESI (Electrospray Ionization) mass spectrometry, that is, "ESIMS/EL guest method".(1) As the results of "FABMS/EL guest method", it was concluded that various chiral crown ethers, carbohydrates, and antibiotics exhibit various degree of chiral recognition toward various amino acids their esters within the range of IRIS=5.0-0.2.(2) As the results of "ESIMS/EL guest method", it was conculuded that ESIMS provides certain depressed IRIS values compared with FABMS so that cautions are required for evaluating chiral recognition ability in a quantitative fashion.